製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Rex1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Rex1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Hues7 and HEK293 whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab125236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

ターゲット情報

  • 機能
    Involved in self-renewal property of ES cells (By similarity). May be involved in transcriptional regulation.
  • 組織特異性
    Expressed in kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells (at protein level). Expressed in malignant kidney and several carcinoma cell lines (at protein level). Expressed in embryonic stem cells, kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells. Expressed in embryonal carcinomas, seminomas, malignant kidney and several carcinoma cell lines.
  • 配列類似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hREX-1 antibody
    • Reduced Expression 1 Protein antibody
    • Reduced expression protein 1 antibody
    • Rex-1 antibody
    • REX1 antibody
    • REX1 transcription factor antibody
    • Zfp-42 antibody
    • Zfp42 antibody
    • ZFP42 zinc finger protein antibody
    • ZFP42_HUMAN antibody
    • Zinc finger protein 42 homolog antibody
    • Zinc finger protein 754 antibody
    • ZNF754 antibody
    see all

画像

  • All lanes : Anti-Rex1 antibody (ab125236) at 1 µg/ml

    Lane 1 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 148 kDa,42 kDa,71 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125236 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

     

    Secondary antibody - goat anti-rabbit HRP (ab97051)

プロトコール

参考文献

ab125236 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Human Cell lysate - whole cell (Undifferentiated human iPS cells)
Specification
Undifferentiated human iPS cells
Blocking step
5% milk in TBST as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Jan 22 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
1% serum, 0.1% triton, 0.1% BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Human Cell (Pluripotent human ES cell (HUES7) on MEFs)
Specification
Pluripotent human ES cell (HUES7) on MEFs
Permeabilization
Yes - see blocking buffer
Fixative
Paraformaldehyde
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投稿 Sep 03 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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