Anti-Retinoid X Receptor gamma 抗体 (ab15518)

製品の概要

  • 製品名
    Anti-Retinoid X Receptor gamma antibody
    Retinoid X Receptor gamma 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Retinoid X Receptor gamma
  • アプリケーション
    適用あり: IHC-Fr, IHC - Wholemount, ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Hamster, Human
  • 免疫原

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Mouse) (N terminal).

  • ポジティブ・コントロール
    • Breast carcinoma and prostate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/200.
IHC - Wholemount Use at an assay dependent concentration.
ICC/IF 1/100.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 51 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid.
  • 配列類似性
    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • ドメイン
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC109416 antibody
    • NR2B3 antibody
    • Nuclear receptor subfamily 2 group B member 3 antibody
    • OTTHUMP00000060418 antibody
    • Retanoic X receptor gamma antibody
    • Retinoic acid receptor RXR gamma antibody
    • Retinoic acid receptor RXR-gamma antibody
    • Retinoid X receptor gamma antibody
    • RXR G antibody
    • RXR gamma antibody
    • RXRC antibody
    • Rxrg antibody
    • RXRG_HUMAN antibody
    • RXRgamma antibody
    see all

画像

  • IHC - Wholemount of Chicken inner ear tissue labelling Retinoid X Receptor gamma with ab15518. Sample was incubated with primary antibody (1/500 in 0.2% Triton-X in PBS) for 12 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab15518 staining Retinoid X Receptor gamma in breast carcinoma by Immunohistochemistry (FFPE-sections).
  • Immunostaining of Retinoid X receptor gamma in adult mouse retina using ab15518. Visible are the outer nuclear layer (top) and inner nuclear layer (bottom) of a adult mouse retina. Retionoid X receptor gamma staining was observed in the cone photoreceptors in the outer nuclear layer and also in most if not all cells of the inner nuclear layer. Please see abreview for protocol details.

    See Abreview

  • Anti-Retinoid X Receptor gamma antibody (ab15518) at 1/25 dilution + NIH3T3 cell lysate

    Predicted band size : 51 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

参考文献

This product has been referenced in:
  • de la Fuente AG  et al. Vitamin D receptor-retinoid X receptor heterodimer signaling regulates oligodendrocyte progenitor cell differentiation. J Cell Biol 211:975-85 (2015). Read more (PubMed: 26644513) »
  • Nickkho-Amiry M  et al. Peroxisome proliferator-activated receptors modulate proliferation and angiogenesis in human endometrial carcinoma. Mol Cancer Res 10:441-53 (2012). WB, IHC-P ; Human . Read more (PubMed: 22205725) »

See all 4 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole brain tissue lysate)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (3-8% Bis-Tris Gels)
Loading amount
10 µg
Specification
Whole brain tissue lysate
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Mar 14 2017

Application
Western blot
Loading amount
50000 cells
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - whole cell (Schwann cells)
Specification
Schwann cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Feb 27 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Heat-inactivated normal donkey serum in 0.05% PBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Mouse, whole brain sections)
Specification
Mouse, whole brain sections
Permeabilization
Yes - Tween-20
Fixative
Paraformaldehyde
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投稿 Jan 07 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Chicken Tissue (Inner Ear Tissue)
Specification
Inner Ear Tissue
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投稿 Jun 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (adult retina)
Specification
adult retina
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton-x100
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
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投稿 Feb 19 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (RXRg expression in U2OS)
Specification
RXRg expression in U2OS
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % Triton X-100
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Mahesh Mathrubutham

Verified customer

投稿 Feb 26 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (CHO K1 cells expressing Human Retinoid X Receptor)
Specification
CHO K1 cells expressing Human Retinoid X Receptor
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % Triton X-100
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Mahesh Mathrubutham

Verified customer

投稿 Sep 05 2007

I'm very sorry to hear you are experiencing problems with our RXR and RAR antibodies, thank you for sending us an image of your blots, I understand your problem. The difference in MW can be slightly different between predicted and detected MW and w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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