Anti-Retinoid X Receptor alpha 抗体 [EPR7106] (ab125001)


  • 製品名Anti-Retinoid X Receptor alpha antibody [EPR7106]
    Retinoid X Receptor alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR7106] to Retinoid X Receptor alpha
  • アプリケーション適用あり: ICC, WB, IPmore details
    適用なし: Flow Cyt or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues at the N terminus of Human Retinoid X Receptor alpha.

  • ポジティブ・コントロール
    • MCF7, HeLa, K562, RAW 264.7, PC12 and NIH 3T3 cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Retinoid X Receptor alpha antibody (HRP) [EPR7106] (ab200782)



Our Abpromise guarantee covers the use of ab125001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/100 - 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).
IP 1/10 - 1/100.
  • 追加情報Is unsuitable for Flow Cyt or IHC-P.
  • ターゲット情報

    • 機能Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
    • 組織特異性Highly expressed in liver, also found in lung, kidney and heart.
    • 配列類似性Belongs to the nuclear hormone receptor family. NR2 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • ドメインComposed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain).
    • 翻訳後修飾Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
      Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FLJ00280 antibody
      • FLJ00318 antibody
      • FLJ16020 antibody
      • FLJ16733 antibody
      • MGC102720 antibody
      • NR2B1 antibody
      • Nuclear receptor subfamily 2 group B member 1 antibody
      • OTTHUMP00000022510 antibody
      • Retinoic acid receptor RXR alpha antibody
      • Retinoic acid receptor RXR-alpha antibody
      • Retinoid X nuclear receptor alpha antibody
      • Retinoid X receptor alpha antibody
      • RXR alpha1 antibody
      • Rxra antibody
      • RXRA_HUMAN antibody
      • RXRalpha1 antibody
      see all

    Anti-Retinoid X Receptor alpha antibody [EPR7106] 画像

    • All lanes : Anti-Retinoid X Receptor alpha antibody [EPR7106] (ab125001) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : RAW 264.7 cell lysate
      Lane 5 : PC12 cell lysate
      Lane 6 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 51 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Retinoid X Receptor alpha antibody [EPR7106] (ab125001) 使用論文

    ab125001 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (Ovarian granulosa)
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris gel)
    Loading amount 500000 cells
    Specification Ovarian granulosa
    Blocking step Odyssey Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C

    Dr. S Rice

    Verified customer

    投稿 Oct 19 2015

    Application Immunohistochemistry (Frozen sections)
    Blocking step 10% Donkey serum + 10% Roche blocking solution (casein-based) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Sample Mouse Tissue sections (Multiple embryonic tissues (liver, heart, intestin)
    Specification Multiple embryonic tissues (liver, heart, intestin
    Permeabilization Yes - 0.3% Triton
    Fixative both snap-frozen unfixed and 4% paraformaldehyde-fixed (pH7.4, 24h)

    Dr. Yaacov Barak

    Verified customer

    投稿 Feb 13 2015