製品の概要

  • 製品名Anti-Retinoid X Receptor alpha antibody
    Retinoid X Receptor alpha 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Retinoid X Receptor alpha
  • 特異性This does not cross-react with either RXR beta or gamma.
  • アプリケーション適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    (C)QVNSSLTSP TGRGSM

    , corresponding to N terminal amino acids 14-28 of Human Retinoid X Receptor alpha.

  • ポジティブ・コントロール
    • Human epidermoid carcinoma A431 lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab24363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 22205725
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
ELISA 1/16000.

ターゲット情報

  • 機能Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.
  • 組織特異性Highly expressed in liver, also found in lung, kidney and heart.
  • 配列類似性Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • ドメインComposed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain).
  • 翻訳後修飾Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
    Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ00280 antibody
    • FLJ00318 antibody
    • FLJ16020 antibody
    • FLJ16733 antibody
    • MGC102720 antibody
    • NR2B1 antibody
    • Nuclear receptor subfamily 2 group B member 1 antibody
    • OTTHUMP00000022510 antibody
    • Retinoic acid receptor RXR alpha antibody
    • Retinoic acid receptor RXR-alpha antibody
    • Retinoid X nuclear receptor alpha antibody
    • Retinoid X receptor alpha antibody
    • RXR alpha1 antibody
    • Rxra antibody
    • RXRA_HUMAN antibody
    • RXRalpha1 antibody
    see all

Anti-Retinoid X Receptor alpha antibody 画像

  • Anti-Retinoid X Receptor alpha antibody (ab24363) at 0.3 µg/ml + Nuclear HeLa lysate at 35 µg

    Predicted band size : 51 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Retinoid X Receptor alpha antibody (ab24363) 使用論文

This product has been referenced in:
  • Nickkho-Amiry M  et al. Peroxisome proliferator-activated receptors modulate proliferation and angiogenesis in human endometrial carcinoma. Mol Cancer Res 10:441-53 (2012). WB, IHC-P ; Human . Read more (PubMed: 22205725) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Gel Running Conditions Reduced Denaturing (NuPage Bis-Tris 4-12% gradient)
Loading amount 40 µg
Specification liver
Blocking step Odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. David Rudnick

Verified customer

投稿 Aug 16 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"