Anti-Retinoic Acid Receptor beta 抗体 [EPR2017] (ab124701)

製品の概要

  • 製品名Anti-Retinoic Acid Receptor beta antibody [EPR2017]
    Retinoic Acid Receptor beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2017] to Retinoic Acid Receptor beta
  • アプリケーション適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Retinoic Acid Receptor beta aa 400 to the C-terminus.

  • ポジティブ・コントロール
    • Human kidney tissue; MCF7, MDA-MB-435, Daudi and U-87 MG cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-Retinoic Acid Receptor beta antibody (HRP) [EPR2017] (ab198557)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
    • 関連疾患Microphthalmia, syndromic, 12
    • 配列類似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • ドメインComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • 細胞内局在Cytoplasm and Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • HAP antibody
      • HBV-activated protein antibody
      • NR1B2 antibody
      • Nuclear receptor subfamily 1 group B member 2 antibody
      • RAR B antibody
      • RAR beta antibody
      • RAR epsilon antibody
      • RAR-beta antibody
      • RAR-epsilon antibody
      • RARB antibody
      • RARB_HUMAN antibody
      • Retinoic acid receptor beta 2 antibody
      • Retinoic acid receptor beta 4 antibody
      • Retinoic acid receptor beta 5 antibody
      • Retinoic acid receptor beta antibody
      • Retinoic acid receptor beta polypeptide antibody
      • RRB2 antibody
      see all

    Anti-Retinoic Acid Receptor beta antibody [EPR2017] 画像

    • All lanes : Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701) at 1/1000 dilution

      Lane 1 : MCF7 cell lysates
      Lane 2 : MDA-MB-435 cell lysates
      Lane 3 : Daudi cell lysates
      Lane 4 : U-87 MG cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lane 1 : Goat anti-Rabbit HRP at 1/20000 dilution
      Lanes 2 - 4 : Goat anti-Rabbit HRP at 1/2000 dilution


      Predicted band size : 50 kDa
    • ab124701 at 1/100 dilution staining Retinoic Acid Receptor beta in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Retinoic Acid Receptor beta antibody [EPR2017] (ab124701) 使用論文

    ab124701 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Adult Testis)
    Specification Adult Testis
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Permeabilization No
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    煜欽 林

    Verified customer

    投稿 Jan 17 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Marmoset (common) Tissue sections (Adult testis)
    Specification Adult testis
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Permeabilization No
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    煜欽 林

    Verified customer

    投稿 Jan 09 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"