Anti-Retinoic Acid Receptor beta 抗体 (ab53161)

製品の概要

  • 製品名Anti-Retinoic Acid Receptor beta antibody
    Retinoic Acid Receptor beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Retinoic Acid Receptor beta
  • アプリケーション適用あり: WB, ELISA, IHC-P, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 351-390 of Human Retinoic Acid Receptor beta

  • ポジティブ・コントロール
    • Human breast carcinoma tissue HepG2 cell extract

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 20655472
ICC/IF Use a concentration of 1 - 5 µg/ml.

ターゲット情報

  • 機能Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
  • 関連疾患Microphthalmia, syndromic, 12
  • 配列類似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • ドメインComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 細胞内局在Cytoplasm and Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HAP antibody
    • HBV-activated protein antibody
    • NR1B2 antibody
    • Nuclear receptor subfamily 1 group B member 2 antibody
    • RAR B antibody
    • RAR beta antibody
    • RAR epsilon antibody
    • RAR-beta antibody
    • RAR-epsilon antibody
    • RARB antibody
    • RARB_HUMAN antibody
    • Retinoic acid receptor beta 2 antibody
    • Retinoic acid receptor beta 4 antibody
    • Retinoic acid receptor beta 5 antibody
    • Retinoic acid receptor beta antibody
    • Retinoic acid receptor beta polypeptide antibody
    • RRB2 antibody
    see all

Anti-Retinoic Acid Receptor beta antibody 画像

  • This image shows human breast carcinoma tissue stained with ab53161 at 1/50 dilution. The left hand image shows untreated tissue; the right hand image shows tissue treated with the immunising peptide.
  • All lanes : Anti-Retinoic Acid Receptor beta antibody (ab53161) at 1/500 dilution

    Lane 1 : HepG2 cell extract, untreated.
    Lane 2 : HepG2 cell extract treated with the immunising peptide.


    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • ICC/IF image of ab53161 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Retinoic Acid Receptor beta antibody (ab53161) 使用論文

This product has been referenced in:
  • Marzese DM  et al. DNA methylation and gene deletion analysis of brain metastases in melanoma patients identifies mutually exclusive molecular alterations. Neuro Oncol N/A:N/A (2014). WB ; Human . Read more (PubMed: 24968695) »
  • Joss-Moore L  et al. Intrauterine growth restriction transiently delays alveolar formation and disrupts retinoic acid receptor expression in the lung of female rat pups. Pediatr Res 73:612-20 (2013). Rat . Read more (PubMed: 23419538) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (adult testis)
Specification adult testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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煜欽 林

Verified customer

投稿 Jan 18 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (marmoset adult testis section)
Specification marmoset adult testis section
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

煜欽 林

Verified customer

投稿 Jan 18 2013

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab53161 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the ...

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