Anti-Retinoic Acid Receptor alpha 抗体 [EP1823Y] (ab76074)

製品の概要

  • 製品名Anti-Retinoic Acid Receptor alpha antibody [EP1823Y]
    Retinoic Acid Receptor alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1823Y] to Retinoic Acid Receptor alpha
  • アプリケーション適用あり: WB, Flow Cytmore details
    適用なし: ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N-terminal of human Retinoic Acid Receptor alpha.

  • ポジティブ・コントロール
    • Permeabilized MCF-7 cells. MCF-7 cell lysate.
  • 特記事項

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab76074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 51 kDa.
Flow Cyt 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ICC,IHC-P or IP.
  • ターゲット情報

    • 機能Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing MLL5. Mediates retinoic acid-induced granulopoiesis.
    • 関連疾患Note=Chromosomal aberrations involving RARA are commonly found in acute promyelocytic leukemia. Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM. The PML-RARA oncoprotein requires both the PML ring structure and coiled-coil domain for both interaction with UBE2I, nuclear microspeckle location and sumoylation. In addition, the coiled-coil domain functions in blocking RA-mediated transactivation and cell differentiation.
    • 配列類似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • ドメインComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • 翻訳後��飾Phosphorylated on serine and threonine residues. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity). Phosphorylation by AKT1 is required for the repressor activity but has no effect on DNA binding, protein stability nor subcellular localization. Phosporylated by PKA in vitro. This phosphorylation on Ser-219 and Ser-369 is critical for ligand binding, nuclear localization and transcriptional activity in response to FSH signaling.
      Sumoylated by SUMO2, mainly on Lys-399 which is also required for SENP6 binding. On all-trans retinoic acid (ATRA) binding, a confromational change may occur that allows sumoylation on two additional site, Lys-166 and Lys-171. Probably desumoylated by SENP6. Sumoylation levels determine nuclear localization and regulate ATRA-mediated transcriptional activity.
      Trimethylation enhances heterodimerization with RXRA and positively modulates the transcriptional activation.
      Ubiquitinated.
    • 細胞内局在Nucleus. Cytoplasm. Nuclear localization depends on ligand binding, phosphorylation and sumoylation. Transloaction to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation.
    • Information by UniProt
    • 参照データベース
    • 別名
      • NR1B1 antibody
      • Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein antibody
      • Nuclear receptor subfamily 1 group B member 1 antibody
      • Nucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form antibody
      • RAR alpha antibody
      • RAR antibody
      • RAR-alpha antibody
      • rara antibody
      • RARA_HUMAN antibody
      • RARalpha antibody
      • RARalpha1 antibody
      • Retinoic acid nuclear receptor alpha variant 1 antibody
      • Retinoic acid nuclear receptor alpha variant 2 antibody
      • Retinoic acid receptor alpha antibody
      • Retinoic acid receptor alpha polypeptide antibody
      see all

    Anti-Retinoic Acid Receptor alpha antibody [EP1823Y] 画像

    • Flow cytometric analysis of permeabilized MCF-7 cells using ab76074 at 1/50 dilution (red) or a rabbit IgG (negative) (green).
    • Anti-Retinoic Acid Receptor alpha antibody [EP1823Y] (ab76074) at 1/1000 dilution + MCF-7 cell lysate at 10 µg

      Predicted band size : 51 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Anti-Retinoic Acid Receptor alpha antibody [EP1823Y] (ab76074) 使用論文

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 100 µg
    Gel Running Conditions Reduced Denaturing (10% Tris-Glycine)
    Sample Hamster Cell lysate - whole cell (BHK cells)
    Specification BHK cells
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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    投稿 Feb 16 2015

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (10% Tris-Glycine)
    Sample Mouse Cell lysate - whole cell (NIH-3T3 cells)
    Specification NIH-3T3 cells
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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    投稿 Feb 13 2015

    Application Western blot
    Loading amount 75 µg
    Gel Running Conditions Reduced Denaturing (10% Tris-Glycine)
    Sample Human Cell lysate - whole cell (HEK293T cells)
    Specification HEK293T cells
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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    投稿 Feb 13 2015

    Application Western blot
    Loading amount 100 µg
    Gel Running Conditions Reduced Denaturing (10% Tris-Glycine)
    Sample Cow Cell lysate - whole cell (MDBK cells)
    Specification MDBK cells
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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    投稿 Feb 11 2015

    The antibody has not been tested for reactivity with RAR beta or RAR gamma. The immunogen that the antibody is raised against is derived from RAR alpha. The amino acid sequence is not conserved in either RAR beta or gamma, so we do not expect the antib...

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    Thank you for contacting us. The concentration of lot GR1841 is 0.376 mg/mL I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Thank you for contacting us. Your credit note ID are: Order No: 896459 - Credit note: 188XX Order No: 944015 - Credit note: 188XX Order No: 955405 - Credit note: 188XX I am sorry that this antibody did not perform as stated on the ...

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    Thank you for your response. As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a ...

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    Thank you for getting back to me and resending the WB images. It looks that the major band(s) are around 40 kDa slightly lower than the expected 50 kDa. These lower bands could either represent modified (processed Retinoic Acid Receptor alpha) or...

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    Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am reviewing your enquiries but unfortunately without knowing what exactly MW markers represent it is difficult to assess the ban...

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