Anti-Retinoblastoma binding protein 6 抗体 (ab55787)


  • 製品名Anti-Retinoblastoma binding protein 6 antibody
    Retinoblastoma binding protein 6 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Retinoblastoma binding protein 6
  • アプリケーション適用あり: Flow Cyt, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1582-1692 of Human Retinoblastoma binding protein 6



Our Abpromise guarantee covers the use of ab55787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.1-1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 3 µg/ml.
WB Use a concentration of 1 - 5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


  • 機能E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome. May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth.
  • 組織特異性Highly expressed in the placenta and testis. Expressed at lower levels in the brain, heart, kidney, liver and lung. Overexpressed in esophageal cancer.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Contains 1 CCHC-type zinc finger.
    Contains 1 DWNN domain.
    Contains 1 RING-type zinc finger.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated by NEK6.
  • 細胞内局在Nucleus > nucleolus. Chromosome. Cytoplasm > cytoskeleton > centrosome. Colocalizes with mitotic chromosomes. Co-localizes with NEK6 in the centrosome.
  • Information by UniProt
  • 参照データベース
  • 別名
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    • P2P R antibody
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    • p53 associated cellular protein of testis antibody
    • p53-associated cellular protein of testis antibody
    • p53-associated cellular protein, testis-derived antibody
    • PACT antibody
    • Proliferation potential related protein antibody
    • Proliferation potential-related protein antibody
    • Protein P2P R antibody
    • Protein P2P-R antibody
    • Protein P2PR antibody
    • Protein RBQ 1 antibody
    • Protein RBQ1 antibody
    • RB binding protein 6, ubiquitin ligase antibody
    • RB binding Q protein 1 antibody
    • RBBP 6 antibody
    • Rbbp6 antibody
    • RBBP6_HUMAN antibody
    • RBQ 1 antibody
    • RBQ-1 antibody
    • RBQ1 antibody
    • Retinoblastoma binding protein 6 isoform 3 antibody
    • Retinoblastoma binding Q protein 1 antibody
    • Retinoblastoma-binding protein 6 antibody
    • Retinoblastoma-binding Q protein 1 antibody
    • SNAMA antibody
    • SNAMA, Drosophila, homolog of antibody
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Anti-Retinoblastoma binding protein 6 antibody 画像

  • Overlay histogram showing Y79 cells stained with ab55787 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55787, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Retinoblastoma binding protein 6 antibody (ab55787) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.
  • Western blot against tagged recombinant protein immunogen using ab55787 Retinoblastoma binding protein 6 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa

Anti-Retinoblastoma binding protein 6 antibody (ab55787) 使用論文

ab55787 has not yet been referenced specifically in any publications.

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