製品の概要

  • 製品名Anti-Reticulon 1A antibody [MON161]
    Reticulon 1A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MON161] to Reticulon 1A
  • 特異性This antibody exclusively recognizes the 135 kD Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues
  • アプリケーション適用あり: IHC-P, WB, IHC-Fr, ICC, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Hamster, Human
  • 免疫原

    Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776).

  • エピトープThe epitope of this antibody is located between amino acid residues 174-337 of Reticulon-1A.
  • 特記事項


    A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 一次抗体 備考A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.
  • ポリ/モノモノクローナル
  • クローン名MON161
  • ミエローマunknown
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.

Recommended range is 1/50 - 1/100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

WB 1/50 - 1/500.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性Reticulon 1A may be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. It is expressed in neural and neuroendocrine tissues and cell cultures derived therefrom. Expression of isoform RTN1-C is strongly correlated with neuronal differentiation. Different isoforms have been described as a result of alternative splicing.
  • 細胞内局在Endoplasmic reticulum
  • 参照データベース
  • 別名
    • Neuroendocrine specific protein antibody
    • NSP antibody
    • Reticulon1 antibody
    • RTN1 antibody

Anti-Reticulon 1A antibody [MON161] (ab9275) 使用論文

This product has been referenced in:
  • Hens J  et al. Neuronal differentiation is accompanied by NSP-C expression. Cell Tissue Res 292:229-37 (1998). Read more (PubMed: 9560466) »
  • Senden NH  et al. A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers. J Pathol 182:13-21 (1997). Read more (PubMed: 9227337) »

See all 5 Publications for this product

Product Wall

Thank you for your enquiry. This target will stain normal and pathological neural and neuroendocrine tissues. To the best of our knowledge there is not a specific protocol that needs to be employed to use this product in either IHC-P or IHC-Fr, you wil...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"