製品の概要

  • 製品名
    Anti-Reticulon 1A antibody [MON160]
    Reticulon 1A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MON160] to Reticulon 1A
  • 特異性
    This antibody exclusively recognizes the 135 kDa Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues
  • アプリケーション
    適用あり: IHC-P, Flow Cyt, ICC, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Hamster, Human
  • 免疫原

    Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776).

  • エピトープ
    This antibody is reactive with residues 174-337 of Reticulon-1A.
  • 特記事項


    A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 一次抗体 備考
    A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.
  • ポリ/モノ
    モノクローナル
  • クローン名
    MON160
  • ミエローマ
    unknown
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.

Recommended range is 1/50 - 1/100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Flow Cyt 1/50 - 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/50 - 1/500.

ターゲット情報

  • 関連性
    Reticulon 1A may be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. It is expressed in neural and neuroendocrine tissues and cell cultures derived therefrom. Expression of isoform RTN1-C is strongly correlated with neuronal differentiation. Different isoforms have been described as a result of alternative splicing.
  • 細胞内局在
    Endoplasmic reticulum
  • 参照データベース
  • 別名
    • Neuroendocrine specific protein antibody
    • NSP antibody
    • Reticulon1 antibody
    • RTN1 antibody

画像

  • NSP-A immunostaining in human cerebellum using ab8957.

  • Human carcinoid (A) and small cell lung carcinoma (B) in paraffin sections, immunostained for NSP-A with ab8957.

参考文献

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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