Anti-Reticular Fibroblasts and Reticular Fibres 抗体 [ER-TR7] (ab51824)

製品の概要

  • 製品名Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7]
  • 製品の詳細
    Rat monoclonal [ER-TR7] to Reticular Fibroblasts and Reticular Fibres
  • アプリケーション適用あり: Flow Cyt, IHC-Frmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Murine thymic reticulum

  • エピトープThe antigen has not been not fully characterized. The recognized epitope may be part of reticulin.
  • ポジティブ・コントロール
    • Mouse spleen, reticular fibroblasts and reticular fibres, Human tonsil.
  • 特記事項Antigen Distribution: Isolated cells: The antigen is found in the cytoplasm of fibroblasts and fibroblast cell lines. Tissue sections: In all organs tested so far, the antigen is expressed in connective tissues which form a supporting network between parenchymal cells (see table below). Thus, the supportive mesenchymal structures of larger vessels can be studied. In spleen, a very clear delineation of red and white pulp is obtained. Capsule, sinuses, follicules, paracortex and medullary cords are also clearly delineated in lymph nodes.


    This monoclonal antibody [ER-TR7] detects an antigen present in, and produced by, reticular fibroblasts. The antibody is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. The antibody also stains subendothelial deposits in the plaque area of atherosclerotic plaques.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS, 5mg/ml BSA, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • 一次抗体 備考This monoclonal antibody [ER-TR7] detects an antigen present in, and produced by, reticular fibroblasts. The antibody is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. The antibody also stains subendothelial deposits in the plaque area of atherosclerotic plaques.
  • ポリ/モノモノクローナル
  • クローン名ER-TR7
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab51824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 2µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr Use a concentration of 1 - 4 µg/ml.
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 関連性Connective tissue consists of a ground of glycosaminoglycans, proteoglycans and glycoproteins through which various fibres run. These fibres can be collagenous, elastic or reticular. Reticular fibres are composed from the family of collagen proteins and give tensile strength. These fibres are made by reticular fibroblasts.

    Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7] 画像

    • ab51824 at 1 µg/ml staining mouse spleen; frozen section.
    • ab51824 staining Reticular Fibroblasts and Reticular Fibres in Mouse spleen tissue sections by Immunohistochemistry (Frozen sections). The sections were fixed with 4% PFA for 10 minutes at room temperature prior to blocking with 10% Goat serum for 30 minutes at 15°C. The primary antibody was diluted 1/500 in PBS with 2% Goat serum and incubated with the sample for 1 hour at 15°C. An Alexa Fluor® 488-conjugated Goat anti-Rat IgG polyclonal was used as the secondary antibody, diluted 1/500.

      See Abreview

    • Overlay histogram showing HT1080 cells stained with ab51824 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51824 , 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758](ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7] (ab51824) 使用論文

    This product has been referenced in:
    • Poduri A  et al. Fibroblast Angiotensin II Type 1a Receptors Contribute to Angiotensin II-Induced Medial Hyperplasia in the Ascending Aorta. Arterioscler Thromb Vasc Biol 35:1995-2002 (2015). IHC-Fr ; Mouse . Read more (PubMed: 26160957) »
    • Thatcher SE  et al. Exogenous 17-ß estradiol administration blunts progression of established angiotensin II-induced abdominal aortic aneurysms in female ovariectomized mice. Biol Sex Differ 6:12 (2015). IHC-Fr ; Mouse . Read more (PubMed: 26131353) »

    See all 15 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (skin)
    Permeabilization No
    Specification skin
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jun 16 2016

    Application Immunohistochemistry free floating
    Sample Mouse Tissue sections (Meninges-Dura Mater)
    Specification Meninges-Dura Mater
    Username

    Abcam user community

    Verified customer

    投稿 Apr 18 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Sample Pig Cultured Cells (kidney)
    Specification kidney
    Permeabilization No
    Fixative Formaldehyde
    Username

    Dr. Zheng Yu Wang

    Verified customer

    投稿 Sep 24 2013



    This antibody reacts with porcine tissue. However, the sequence is not known.

    Thank you for your enquiry and your interest in our products.

    The tested applications and the species are normally listed on the on-line product datasheets. Generally, we test our antibodies in human, mouse and rat samples - very few antibodi...

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    Thank you for your enquiry.

    I can confirm that ab51824 has been tested on frozen section sections (IHC-Fr), so would be covered by our guarantee for frozen sections in IF.

    However, as far as we are aware, ithas never been tested i...

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    Thank you for contacting us. I would recommend the Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7] ab51824 (www.abcam.com/ab51824) since this product does not react with keratinocytes. As shown on the datasheet it has been tested in ...

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    L'anticorps monoclonal ab51824, clone [ER-TR7], reconnait un antigène présent et produit par les fibroblasts réticulaires. Cet anticorps permet le marquage spécifique du tissu conjonctif des organes lymphoïdes.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (spleen)
    Specification spleen
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 15°C
    Username

    Dr. Lynda Coughlan

    Verified customer

    投稿 Dec 15 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Cell (skin)
    Specification skin
    Fixative Acetone
    Permeabilization No
    Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 20 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"