製品の概要

  • 製品名Anti-Ret antibody
    Ret 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ret
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Ret.

    (Peptide available as ab49595.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in LoVo (Human colon adenocarcinoma cell line) Nuclear lysate and K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate - tumor cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 119 kDa (predicted molecular weight: 124 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Probable receptor with tyrosine-protein kinase activity; important for development.
    • 関連疾患Defects in RET may be a cause of colorectal cancer (CRC) [MIM:114500].
      Defects in RET are a cause of Hirschsprung disease (HSCR) [MIM:142623]. HSCR is a genetic disorder of neural crest development characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Occasionally, MEN2A or FMTC occur in association with HSCR.
      Defects in RET are the cause of medullary thyroid carcinoma (MTC) [MIM:155240]. MTC is a rare tumor derived from the C cells of the thyroid. Three hereditary forms are known, that are transmitted in an autosomal dominant fashion: (a) multiple neoplasia type 2A (MEN2A), (b) multiple neoplasia type IIB (MEN2B) and (c) familial MTC (FMTC), which occurs in 25-30% of MTC cases and where MTC is the only clinical manifestation.
      Defects in RET are the cause of multiple neoplasia type 2B (MEN2B) [MIM:162300]. MEN2B is an uncommon inherited cancer syndrome characterized by predisposition to MTC and phaeochromocytoma which is associated with marfanoid habitus, mucosal neuromas, skeletal and ophtalmic abnormalities, and ganglioneuromas of the intestine tract. Then the disease progresses rapidly with the development of metastatic MTC and a pheochromocytome in 50% of cases.
      Defects in RET are a cause of susceptibility to pheochromocytoma (PCC) [MIM:171300]. A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent.
      Defects in RET are the cause of multiple neoplasia type 2A (MEN2A) [MIM:171400]; also known as multiple neoplasia type 2 (MEN2). MEN2A is the most frequent form of medullary thyroid cancer (MTC). It is an inherited cancer syndrome characterized by MTC, phaeochromocytoma and/or hyperparathyroidism.
      Defects in RET are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving RET are found in thyroid papillary carcinomas. Inversion inv(10)(q11.2;q21) generates the RET/CCDC6 (PTC1) oncogene; inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene; translocation t(10;14)(q11;q32) with GOLGA5 generates the RET/GOLGA5 (PTC5) oncogene; translocation t(8;10)(p21.3;q11.2) with PCM1 generates the PCM1/RET fusion; translocation t(6;10)(p21.3;q11.2) with RFP generates the Delta RFP/RET oncogene; translocation t(1;10)(p13;q11) with TRIM33 generates the TRIM33/RET (PTC7) oncogene; translocation t(7;10)(q32;q11) with TRIM24/TIF1 generates the TRIM24/RET (PTC6) oncogene. The PTC5 oncogene has been found in 2 cases of PACT in children exposed to radioactive fallout after Chernobyl. A chromosomal aberration involving TRIM27/RFP is found in thyroid papillary carcinomas. Translocation t(6;10)(p21.3;q11.2) with RET. The translocation generates TRIM27/RET and delta TRIM27/RET oncogenes.
      Defects in RET are a cause of renal adysplasia (RADYS) [MIM:191830]; also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy.
      Defects in RET are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
    • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family.
      Contains 1 cadherin domain.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Autophosphorylated on C-terminal tyrosine residues upon ligand stimulation. Dephosphorylated by PTPRJ on Tyr-905, Tyr-1015 and Tyr-1062.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C ret antibody
      • Cadherin family member 12 antibody
      • Cadherin related family member 16 antibody
      • CDHF 12 antibody
      • CDHF12 antibody
      • CDHR16 antibody
      • ELKS Fusion gene antibody
      • HSCR 1 antibody
      • HSCR1 antibody
      • Hydroxyaryl protein kinase antibody
      • MEN2A antibody
      • MEN2B antibody
      • MTC 1 antibody
      • MTC1 antibody
      • Multiple endocrine neoplasia and medullary thyroid carcinoma 1 antibody
      • Oncogene RET antibody
      • Proto oncogene tyrosine protein kinase receptor ret antibody
      • Proto-oncogene c-Ret antibody
      • Proto-oncogene tyrosine-protein kinase receptor ret antibody
      • PTC antibody
      • RET antibody
      • RET ELE1 antibody
      • Ret Proto oncogene antibody
      • RET transforming sequence antibody
      • RET_HUMAN antibody
      • RET51 antibody
      • RET9 antibody
      • tyrosine-protein kinase receptor ret antibody
      see all

    Anti-Ret antibody 画像

    • All lanes : Anti-Ret antibody (ab49596) at 1 µg/ml

      Lane 1 : LoVo nuclear extract lysate (ab14859)
      Lane 2 : K562 whole cell lysate (ab29306)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 124 kDa
      Observed band size : 119 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 40 kDa,53 kDa. We are unsure as to the identity of these extra bands.

    Anti-Ret antibody (ab49596) 使用論文

    ab49596 has not yet been referenced specifically in any publications.

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