製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to REST / NRSF
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Vector coding for a partial recombinant fusion protein, corresponding to internal sequence amino acids 210-310 of Human REST/ NRSF. Target sequence used to make antibody: DFSKGPIRCD RCGYNTNRYD HYTAHLKHHT RAGDNERVYK CIICTYTTVS EYHWRKHLRN HFPRKVYTCG KCNYFSDRKN NYVQHVRTHT GERPYKCELC.

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度
    Whole antiserum
  • 一次抗体 備考
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 132 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能
    Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
  • 組織特異性
    Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
  • 配列類似性
    Contains 9 C2H2-type zinc fingers.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Neural Restrictive Silencer Factor antibody
    • Neural-restrictive silencer factor antibody
    • Neuron Restrictive Silencer Factor antibody
    • NRSF antibody
    • RE1 silencing transcription factor antibody
    • RE1-silencing transcription factor antibody
    • Repressor binding to the X2 box antibody
    • rest antibody
    • REST protein antibody
    • REST_HUMAN antibody
    • X2 box repressor antibody
    • XBR antibody
    see all

Anti-REST / NRSF antibody 画像

  • All lanes : Anti-REST / NRSF antibody (ab52849) at 1/1000 dilution

    Lane 1 : Total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
    Lane 2 : Total protein extract from E coli with 50ng to 500ng of the antigen (Tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, HRP conjugated, at 1/5000 dilution

    Predicted band size : 132 kDa

プロトコール

Anti-REST / NRSF antibody (ab52849) 使用論文

ab52849 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
90 µg
Gel Running Conditions
Reduced Denaturing (4-18% gel)
Sample
Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
Specification
Mouse embryonic stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 17 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録