Anti-Respiratory Syncytial Virus M2-1 Protein 抗体 [RSV5H5] (ab94805)
Key features and details
- Mouse monoclonal [RSV5H5] to Respiratory Syncytial Virus M2-1 Protein
- Suitable for: ELISA, WB, IP, ICC/IF
- Reacts with: Respiratory syncytial virus
- Isotype: IgG1
製品の概要
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製品名
Anti-Respiratory Syncytial Virus M2-1 Protein antibody [RSV5H5] -
製品の詳細
Mouse monoclonal [RSV5H5] to Respiratory Syncytial Virus M2-1 Protein -
由来種
Mouse -
特異性
This antibody has broad specificity for Respiratory Syncytial Virus of both sub-groups A and B -
アプリケーション
適用あり: ELISA, WB, IP, ICC/IFmore details -
種交差性
交差種: Respiratory syncytial virus -
免疫原
Tissue, cells or virus corresponding to Respiratory Syncytial Virus M2-1 Protein. Respiratory Syncytial Virus strain A2 infected HeLa cells.
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特記事項
Fusion partner: PS-NS/1-Ag4 Recommended growth conditions: Growth medium RPMI with 25mM Hepes, 2mM additional glutamine and 10% heat inactivated foetal calf serumThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
RSV5H5 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94805の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
on Acetone fixed or Paraffin embedded samples. |
特記事項 |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. on Acetone fixed or Paraffin embedded samples. |
ターゲット情報
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関連性
The M2-1 protein is an essential element in the RSV replication complex. -
細胞内局在
viral envelope -
参照データベース
- Entrez Gene: 1494476 Respiratory syncytial virus
- SwissProt: Q76TK9 Respiratory syncytial virus
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別名
- M2/22K antibody
- Matrix antibody
画像
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RSV mutants show expected patterns of G, F, and M2-1 expression.
Western blotting of Vero cell lysates infected with RSV ΔG, ΔG C-term, and wild type (MSA1).
(Figure C shown) Detection of M2-1 using anti-M2-1 antibody ab94805.
Cell lysates from virus cultures grown for 6 days on Vero cells were subjected to SDS-PAGE, transferred to membranes and probed with anti-M2-1 antibody (Abcam, ab94805), and incubated with a secondary goat anti-mouse alkaline phosphatase conjugate. Anti-G western blot was done with rabbit anti-RSV-G polyclonal and incubated with goat anti-rabbit secondary antibody (Abcam, ab97048). Bands were visualized using BCIP/NBT. The molecular weight marker used was Precision Plus Protein Dual Color Standards.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab94805 は 10 報の論文で使用されています。
- Braun MR et al. Respiratory syncytial virus M2-1 protein associates non-specifically with viral messenger RNA and with specific cellular messenger RNA transcripts. PLoS Pathog 17:e1009589 (2021). PubMed: 34003848
- Liang W et al. Cyclophilin A Inhibits Human Respiratory Syncytial Virus (RSV) Replication by Binding to RSV-N through Its PPIase Activity. J Virol 95:e0056321 (2021). PubMed: 34011546
- Touzelet O et al. The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins. Mol Cell Proteomics 19:793-807 (2020). PubMed: 32075873
- Blanchard EL et al. Polymerase-tagged respiratory syncytial virus reveals a dynamic rearrangement of the ribonucleocapsid complex during infection. PLoS Pathog 16:e1008987 (2020). PubMed: 33031461
- Patel N et al. Flexible RSV Prefusogenic Fusion Glycoprotein Exposes Multiple Neutralizing Epitopes that May Collectively Contribute to Protective Immunity. Vaccines (Basel) 8:N/A (2020). PubMed: 33066540
- Bouillier C et al. The Interactome analysis of the Respiratory Syncytial Virus protein M2-1 suggests a new role in viral mRNA metabolism post-transcription. Sci Rep 9:15258 (2019). PubMed: 31649314
- Jeong KI et al. CX3CR1 Is Expressed in Differentiated Human Ciliated Airway Cells and Co-Localizes with Respiratory Syncytial Virus on Cilia in a G Protein-Dependent Manner. PLoS One 10:e0130517 (2015). WB ; Respiratory syncytial virus . PubMed: 26107373
- Challa S et al. Mechanism of action for respiratory syncytial virus inhibitor RSV604. Antimicrob Agents Chemother 59:1080-7 (2015). ICC/IF ; Respiratory syncytial virus . PubMed: 25451060
- Kiss G et al. Structural analysis of respiratory syncytial virus reveals the position of M2-1 between the matrix protein and the ribonucleoprotein complex. J Virol 88:7602-17 (2014). Respiratory syncytial virus . PubMed: 24760890
- Fricke J et al. p38 and OGT sequestration into viral inclusion bodies in cells infected with human respiratory syncytial virus suppresses MK2 activities and stress granule assembly. J Virol 87:1333-47 (2013). WB, ICC/IF ; Respiratory syncytial virus . PubMed: 23152511