Anti-Respiratory Syncytial Virus Fusion (F) Glycoprotein 抗体 [RSV5A6] (ab94968)

製品の概要

  • 製品名
    Anti-Respiratory Syncytial Virus Fusion (F) Glycoprotein antibody [RSV5A6]
  • 製品の詳細
    Mouse monoclonal [RSV5A6] to Respiratory Syncytial Virus Fusion (F) Glycoprotein
  • アプリケーション
    適用あり: ELISA, WB, ICC/IF, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Other
  • 免疫原

    Human Respiratory Syncytial Virus strain A2 infected HeLa cells

  • 特記事項
    Fusion partner: PS-NS/1-Ag4


    ab94968 is useful for the identification and location of expression of the fusion glycoprotein of Human Respiratory Syncytial Virus of both sub-groups A and B.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 一次抗体 備考
    ab94968 is useful for the identification and location of expression of the fusion glycoprotein of Human Respiratory Syncytial Virus of both sub-groups A and B.
  • ポリ/モノ
    モノクローナル
  • クローン名
    RSV5A6
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab94968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Fix with Acetone.

ターゲット情報

  • 関連性
    Respiratory Syncytial Virus (RSV) Fusion (F) Glycoprotein is a Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (protein F) interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between host cell and virion membranes. Notably, RSV fusion protein is able to interact directly with heparan sulfate and therefore actively participates in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.
  • 細胞内局在
    Virion membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass membrane protein.
  • 別名
    • F antibody
    • Fusion glycoprotein F0 antibody
    • Protein F antibody
    • RSV Fusion (F) Glycoprotein antibody

参考文献

This product has been referenced in:
  • West WH  et al. Biological activity, binding site and affinity of monoclonal antibodies to the fusion protein of respiratory syncytial virus. J Gen Virol 75 ( Pt 10):2813-9 (1994). Read more (PubMed: 7931172) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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