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The measurement of mitochondrial dehydrogenases (e.g. LDH) activity is a well-accepted assay to quantify cell numbers and monitor cell viability.
ab112119 Cell Cytotoxicity Assay Kit provides a fast, simple, accurate and homogeneous assay for the fluorimetric detection of viable cells. ab112119 is based on the observation that oxidized non-fluorescent blue resazurin is reduced to a red fluorescent dye (resorufin) by accepting an electron from mitochondrial respiratory chain in live cells. The amount of resorufin produced is directly proportional to the number of living cells.
ab112119 is more sensitive for cell proliferation and cytotoxicity than other assays such as MTT. The kit components are quite stable with minimal cytotoxicity, thus a longer incubation time (such as 24-48 hours) is possible if required.
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ab112119 should be stored desiccated
|Assay Solution||1 x 20ml|
Our Abpromise guarantee covers the use of ab112119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab112119 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"