Anti-Repulsive Guidance Molecule C 抗体 (ab104492)

製品の概要

  • 製品名Anti-Repulsive Guidance Molecule C antibody
    Repulsive Guidance Molecule C 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Repulsive Guidance Molecule C
  • アプリケーション適用あり: IHC-Fr, ICC/IF, WB, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-313 of Human Repulsive Guidance Molecule C isoform b (NP_660320.3).

  • ポジティブ・コントロール
    • Mouse spleen. Repulsive Guidance Molecule C transfected 293T cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB 1/500. Predicted molecular weight: 34 kDa.
IHC-FoFr Use at an assay dependent concentration.

ターゲット情報

  • 関連性Mutations in Repulsive Guidance Molecule (also known as RGM-C; HJV; hemojuvelin; Hfe2) cause juvenile hemochromatosis, a severe iron overload disease. RGM-C gene expression has been characterized in the developing mouse and found to be exclusively expressed in all striated muscle and in the myocardium.
  • 細胞内局在Cell membrane; Lipid-anchor, GPI-anchor
  • 参照データベース
  • 別名
    • 2310035L15Rik antibody
    • 5230400G09Rik antibody
    • AI414844 antibody
    • AI789733 antibody
    • DL M antibody
    • Haemojuvelin antibody
    • HEMOCHROMATOSIS antibody
    • HEMOCHROMATOSIS DUE TO DEFECT IN HEMOJUVELIN antibody
    • HEMOCHROMATOSIS DUE TO DEFECT IN HEPCIDIN ANTIMICROBIAL PEPTIDE antibody
    • HEMOCHROMATOSIS JUVENILE antibody
    • Hemochromatosis type 2 (juvenile) antibody
    • Hemochromatosis type 2 antibody
    • Hemochromatosis type 2 protein antibody
    • Hemochromatosis type 2 protein homolog antibody
    • HEMOCHROMATOSIS, TYPE 2A antibody
    • HEMOCHROMATOSIS, TYPE 2B antibody
    • Hemojuvelin antibody
    • HFE 2 antibody
    • Hfe2 antibody
    • HFE2A antibody
    • HJV antibody
    • JH antibody
    • Juvenile antibody
    • MGC23953 antibody
    • OTTHUMP00000059680 antibody
    • Repulsive guidance molecule c antibody
    • RGM C antibody
    • RGM domain family member C antibody
    • RGMC antibody
    see all

Anti-Repulsive Guidance Molecule C antibody 画像

  • Anti-Repulsive Guidance Molecule C antibody (ab104492) at 1/500 dilution + Mouse spleen tissue lysate at 50 µg

    Predicted band size : 34 kDa
  • All lanes : Anti-Repulsive Guidance Molecule C antibody (ab104492) at 1/500 dilution

    Lane 1 : Repulsive Guidance Molecule C transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 34 kDa
  • IHC-FoFr image of ab104492 staining of Repulsive Guidance Molecule C in mouse muscle tissue. The tissue sections were permeabilised with 0.3% TritonX and incubated in 10% serum, with the latter being used to block non-specific protein-protein interactions. The sections were then incubated with ab104492 (1:1000) for 24 hours at +4°C, followed by Alexa Fluor568 conjugated secondary antibody.

    See Abreview

  • ICC/IF image of ab104492 staining of Repulsive Guidance Molecule C in rat myoblast cell culture. The sections were incubated in 0.3% triton X to permeabilise the cells and in 10% serum for 1 hour at 24°C to block non-specific protein-protein interactions. The sections were then incubated with ab104492 (1:1000) for four hours at 24°C, followed by Alexa Fluor568 conjugated secondary antibody.

    See Abreview

Anti-Repulsive Guidance Molecule C antibody (ab104492) 使用論文

ab104492 has not yet been referenced specifically in any publications.

Product Wall

Vielen Dank für Ihren Anruf.

Es tut mir ausgesprochen leid, aber wir haben leider keinen Antikörper, der für Ihre Zwecke geeignet zu sein scheint (alle unsere RGM C scheinen eher mit dem RGM A als mit C in Cynomolgus zu reagieren, jedenfalls...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Acetone
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Abcam user community

Verified customer

投稿 Jul 24 2012

Application Western blot
Sample Rat Tissue lysate - whole (Heart)
Loading amount 50 µg
Specification Heart
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jul 24 2012

Application Western blot
Sample Rat Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Apr 27 2012

Thank you for submitting your review.
I'm sorry to hear this vial of ab28990 is giving you trouble. I just have two additional questions:
1. If you could, please submit a new western blot figure, noting molecular weight markers
2. Do all...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Spleen)
Specification Spleen
Fixative Acetone
Permeabilization Yes - 0,3% Triton-X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Mar 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Muscle, heart, liver)
Loading amount 20 µg
Specification Muscle, heart, liver
Gel Running Conditions Reduced Denaturing (4-12% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Oct 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Myoblast)
Specification Myoblast
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Oct 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Muscle)
Specification Muscle
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Oct 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"