Anti-Reptin/TIP49B/RUVB2 抗体 (ab91462)
Key features and details
- Rabbit polyclonal to Reptin/TIP49B/RUVB2
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Reptin/TIP49B/RUVB2 antibody
Reptin/TIP49B/RUVB2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Reptin/TIP49B/RUVB2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Whole cell lystae from HeLa cells, 293T cells and Mouse NIH3T3 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was affinity purified using an epitope specific to Reptin/TIP49B/RUVB2 immobilized on solid support -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab91462の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 51 kDa.
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IP |
Use at 5-15 µg/mg of lysate.
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特記事項 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Predicted molecular weight: 51 kDa. |
IP
Use at 5-15 µg/mg of lysate. |
ターゲット情報
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機能
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. -
組織特異性
Ubiquitously expressed. Highly expressed in testis and thymus. -
配列類似性
Belongs to the ruvB family. -
ドメイン
The C-terminal domain is required for association with ATF2. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes. - Information by UniProt
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参照データベース
- Entrez Gene: 511048 Cow
- Entrez Gene: 10856 Human
- Entrez Gene: 20174 Mouse
- Entrez Gene: 292907 Rat
- Omim: 604788 Human
- SwissProt: Q2TBU9 Cow
- SwissProt: Q9Y230 Human
- SwissProt: Q9WTM5 Mouse
see all -
別名
- 48 kDa TATA box-binding protein-interacting protein antibody
- 48 kDa TBP-interacting protein antibody
- 48-kDa TATA box-binding protein-interacting protein antibody
see all
画像
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All lanes : Anti-Reptin/TIP49B/RUVB2 antibody (ab91462) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Reptin/TIP49B/RUVB2 with ab91462 at 1/1000 (1µg/ml). Detection: DAB.
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ab91462 at 1 µg/ml detecting Reptin/TIP49B/RUVB2 in HeLa whole cell lysate by WB following IP. Immunoprecipitation utilised ab91462 at 10 µg/mg of lysate (lane 1). Reptin/TIP49B/RUVB2 was also immunoprecipitated by an antibody to an downstream epitope of Reptin/TIP49B/RUVB2 (lane 2) but not by control IgG (lane 3). 1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 30 second exposure.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab91462 は 2 報の論文で使用されています。
- Huizar RL et al. A liquid-like organelle at the root of motile ciliopathy. Elife 7:N/A (2018). PubMed: 30561330
- Li Y et al. Axonemal dynein assembly requires the R2TP complex component Pontin. Development 144:4684-4693 (2017). PubMed: 29113992