製品の概要
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製品名Anti-Reptin/TIP49B/RUVB2 antibody
Reptin/TIP49B/RUVB2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Reptin/TIP49B/RUVB2
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アプリケーション適用あり: WB, IHC-Pmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Recombinant protein (Human)
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ポジティブ･コントロール
- WB: A431 or H1299 cell lysate. IHC-P: skin, testis, or uterus tissue.
法規制情報
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris glycine, pH 7 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab109872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 51 kDa. | |
| IHC-P | Use a concentration of 10 µg/ml. |
ターゲット情報
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機能Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.
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組織特異性Ubiquitously expressed. Highly expressed in testis and thymus.
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配列類似性Belongs to the ruvB family.
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ドメインThe C-terminal domain is required for association with ATF2.
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翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
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細胞内局在Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes.
- Information by UniProt
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参照データベース
- Entrez Gene: 511048 Cow
- Entrez Gene: 10856 Human
- Entrez Gene: 20174 Mouse
- Entrez Gene: 292907 Rat
- Omim: 604788 Human
- SwissProt: Q2TBU9 Cow
- SwissProt: Q9Y230 Human
- SwissProt: Q9WTM5 Mouse
see all -
別名
- 48 kDa TATA box-binding protein-interacting protein antibody
- 48 kDa TBP-interacting protein antibody
- 48-kDa TATA box-binding protein-interacting protein antibody
see all
Anti-Reptin/TIP49B/RUVB2 antibody 画像
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Western blot - Reptin/TIP49B/RUVB2 antibody (ab109872)All lanes : Anti-Reptin/TIP49B/RUVB2 antibody (ab109872) at 1/5000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size : 51 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Reptin/TIP49B/RUVB2 antibody (ab109872)ab109872 at 10 µg/ml staining formalin-fixed, paraffin-embedded Human skin tissue. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Reptin/TIP49B/RUVB2 antibody (ab109872)ab109872 at 10 µg/ml staining formalin-fixed, paraffin-embedded Human testis tissue. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Reptin/TIP49B/RUVB2 antibody (ab109872)ab109872 at 10 µg/ml staining formalin-fixed, paraffin-embedded Human uterus tissue.
Anti-Reptin/TIP49B/RUVB2 antibody (ab109872) 使用論文
ab109872 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"