Anti-RENT1/hUPF1 抗体 (ab92655)


  • 製品名Anti-RENT1/hUPF1 antibody
    RENT1/hUPF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RENT1/hUPF1
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide derived from the internal region of Human RENT1/hUPF1.

  • ポジティブ・コントロール
    • K562 cells, NIH/3T3 cells and Human colon carcinoma tissue.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using an epitope-specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野



Our Abpromise guarantee covers the use of ab92655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
  • 追加情報ELISA: 1/20000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 124 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Plays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability.
    • 組織特異性Ubiquitous.
    • 配列類似性Belongs to the DNA2/NAM7 helicase family.
      Contains 1 C2H2-type zinc finger.
    • ドメインThe [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
    • 翻訳後修飾Phosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Cytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATP dependent helicase RENT1 antibody
      • ATP-dependent helicase RENT1 antibody
      • Delta helicase antibody
      • FLJ43809 antibody
      • FLJ46894 antibody
      • HUPF 1 antibody
      • hUpf1 antibody
      • KIAA0221 antibody
      • Nonsense mRNA reducing factor 1 antibody
      • NORF 1 antibody
      • NORF1 antibody
      • pNORF 1 antibody
      • pNORF1 antibody
      • Regulator of nonsense transcripts 1 antibody
      • RENT 1 antibody
      • RENT1 antibody
      • RENT1_HUMAN antibody
      • Smg 2 antibody
      • Smg 2 homolog nonsense mediated mRNA decay factor antibody
      • UP Frameshift 1 antibody
      • Up frameshift mutation 1 homolog (S. cerevisiae) antibody
      • Up frameshift mutation 1 homolog antibody
      • Up frameshift suppressor 1 homolog antibody
      • Up-frameshift suppressor 1 homolog antibody
      • UPF 1 antibody
      • UPF 1 regulator of nonsense transcripts homolog antibody
      • upf1 antibody
      • UPF1 regulator of nonsense transcripts homolog antibody
      • UPF1 RNA helicase and ATPase antibody
      • Yeast Upf1p homolog antibody
      see all

    Anti-RENT1/hUPF1 antibody 画像

    • All lanes : Anti-RENT1/hUPF1 antibody (ab92655) at 1/500 dilution

      Lane 1 : Extract from NIH/3T3 cells (without competing peptide)
      Lane 2 : Extract from K562 cells (without competing peptide)
      Lane 3 : Extract from NIH/3T3 cells (with competing peptide)

      Lysates/proteins at 5 µg per lane.

      Predicted band size : 124 kDa
    • ab92655, at 1/50 dilution ,staining RENT1/hUPF1 in paraffin-embedded Human colon carcinoma tissue (1) without competing peptide and (2) with competing peptide, by Immunohistochemistry (Formalin/PFA-fixed parafin-embedded sections).

    Anti-RENT1/hUPF1 antibody (ab92655) 使用論文

    ab92655 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting Abcam about ab109363, ab92655 and ab10534. Unfortunately the immunogen sequences for these three antibodies is proprietary information and I do not have access to that information. Please except my apologies for n...

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