製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to RENT1/hUPF1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
  • 免疫原

    Immunogen was a synthetic peptide, to a region between residue 250 and 300 of human Regulator of Nonsense Transcripts 1 (up-frameshift mutation 1 homolog) using the numbering given in entry NP_002902.2 (GeneID 5976).).

  • ポジティブ・コントロール
    • Whole cell lysate from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antibodies were affinity purified using the peptide immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2500. Detects a band of approximately 140 kDa (predicted molecular weight: 134 kDa).

ターゲット情報

  • 機能
    Plays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the DNA2/NAM7 helicase family.
    Contains 1 C2H2-type zinc finger.
  • ドメイン
    The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
  • 翻訳後修飾
    Phosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Cytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATP dependent helicase RENT1 antibody
    • ATP-dependent helicase RENT1 antibody
    • Delta helicase antibody
    • FLJ43809 antibody
    • FLJ46894 antibody
    • HUPF 1 antibody
    • hUpf1 antibody
    • KIAA0221 antibody
    • Nonsense mRNA reducing factor 1 antibody
    • NORF 1 antibody
    • NORF1 antibody
    • pNORF 1 antibody
    • pNORF1 antibody
    • Regulator of nonsense transcripts 1 antibody
    • RENT 1 antibody
    • RENT1 antibody
    • RENT1_HUMAN antibody
    • Smg 2 antibody
    • Smg 2 homolog nonsense mediated mRNA decay factor antibody
    • UP Frameshift 1 antibody
    • Up frameshift mutation 1 homolog (S. cerevisiae) antibody
    • Up frameshift mutation 1 homolog antibody
    • Up frameshift suppressor 1 homolog antibody
    • Up-frameshift suppressor 1 homolog antibody
    • UPF 1 antibody
    • UPF 1 regulator of nonsense transcripts homolog antibody
    • upf1 antibody
    • UPF1 regulator of nonsense transcripts homolog antibody
    • UPF1 RNA helicase and ATPase antibody
    • Yeast Upf1p homolog antibody
    see all

画像



  • Predicted band size : 134 kDa


    ab10510 detection of human RENT1 by Western blot. Samples: Whole cell lysate (30 µg) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody: ab10510 used at 1 µg/ml. Detection: Chemiluminescence.

プロトコール

参考文献

This product has been referenced in:
  • Truong DM  et al. Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation. PLoS Genet 11:e1005422 (2015). Read more (PubMed: 26241656) »
  • Henderson-Smith A  et al. SMG1 identified as a regulator of Parkinson's disease-associated alpha-synuclein through siRNA screening. PLoS One 8:e77711 (2013). WB . Read more (PubMed: 24204929) »

See all 3 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Arabidopsis thaliana Tissue lysate - whole (10 day old seedlings, grown on MS medium)
Gel Running Conditions
Reduced Denaturing (8% gel)
Loading amount
100 µg
Specification
10 day old seedlings, grown on MS medium
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 22 2015

Thank you for contacting Abcam. Having looked at all our RENT1/UPF1 antibodies, I believe that ab10510 would be the most likely to work with your sample. Unfortunately I do not have access to the actual immunogen sequence that was used to gener...

Read More

Thank you for contacting us. We unfortunately do not have antibodies that are tested with Arabidopsis thaliana species. However after checking the immunogen sequence homology it seems that antibody ab10510 immunogen (amino acid 250 to 300) has a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Loading amount
50 µg
Specification
U2OS cells
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycine)
Blocking step
normal horse serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Natalie Farny

Verified customer

投稿 Oct 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録