製品の概要

  • 製品名Anti-RENT1/hUPF1 antibody
    RENT1/hUPF1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to RENT1/hUPF1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Immunogen was a synthetic peptide, to a region between residue 250 and 300 of human Regulator of Nonsense Transcripts 1 (up-frameshift mutation 1 homolog) using the numbering given in entry NP_002902.2 (GeneID 5976).).

  • ポジティブ・コントロール
    • Whole cell lysate from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Antibodies were affinity purified using the peptide immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2500. Detects a band of approximately 140 kDa (predicted molecular weight: 134 kDa).

ターゲット情報

  • 機能Plays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the DNA2/NAM7 helicase family.
    Contains 1 C2H2-type zinc finger.
  • ドメインThe [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
  • 翻訳後修飾Phosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATP dependent helicase RENT1 antibody
    • ATP-dependent helicase RENT1 antibody
    • Delta helicase antibody
    • FLJ43809 antibody
    • FLJ46894 antibody
    • HUPF 1 antibody
    • hUpf1 antibody
    • KIAA0221 antibody
    • Nonsense mRNA reducing factor 1 antibody
    • NORF 1 antibody
    • NORF1 antibody
    • pNORF 1 antibody
    • pNORF1 antibody
    • Regulator of nonsense transcripts 1 antibody
    • RENT 1 antibody
    • RENT1 antibody
    • RENT1_HUMAN antibody
    • Smg 2 antibody
    • Smg 2 homolog nonsense mediated mRNA decay factor antibody
    • UP Frameshift 1 antibody
    • Up frameshift mutation 1 homolog (S. cerevisiae) antibody
    • Up frameshift mutation 1 homolog antibody
    • Up frameshift suppressor 1 homolog antibody
    • Up-frameshift suppressor 1 homolog antibody
    • UPF 1 antibody
    • UPF 1 regulator of nonsense transcripts homolog antibody
    • upf1 antibody
    • UPF1 regulator of nonsense transcripts homolog antibody
    • UPF1 RNA helicase and ATPase antibody
    • Yeast Upf1p homolog antibody
    see all

Anti-RENT1/hUPF1 antibody 画像



  • Predicted band size : 134 kDa


    ab10510 detection of human RENT1 by Western blot. Samples: Whole cell lysate (30 µg) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody: ab10510 used at 1 µg/ml. Detection: Chemiluminescence.

Anti-RENT1/hUPF1 antibody (ab10510) 使用論文

This product has been referenced in:
  • Shi J  et al. Caffeine regulates alternative splicing in a subset of cancer-associated genes: a role for SC35. Mol Cell Biol 28:883-95 (2008). WB ; Human . Read more (PubMed: 18025108) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Arabidopsis thaliana Tissue lysate - whole (10 day old seedlings, grown on MS medium)
Gel Running Conditions Reduced Denaturing (8% gel)
Loading amount 100 µg
Specification 10 day old seedlings, grown on MS medium
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 22 2015

Thank you for contacting Abcam. Having looked at all our RENT1/UPF1 antibodies, I believe that ab10510 would be the most likely to work with your sample. Unfortunately I do not have access to the actual immunogen sequence that was used to gener...

Read More

Thank you for contacting us. We unfortunately do not have antibodies that are tested with Arabidopsis thaliana species. However after checking the immunogen sequence homology it seems that antibody ab10510 immunogen (amino acid 250 to 300) has a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS cells)
Loading amount 50 µg
Specification U2OS cells
Gel Running Conditions Reduced Denaturing (4-20% tris-glycine)
Blocking step normal horse serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Natalie Farny

Verified customer

投稿 Oct 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"