製品の概要

  • 製品名
    Anti-RENBP antibody
  • 製品の詳細
    Rabbit polyclonal to RENBP
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Pig, Baboon
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human RENBP.

    (Peptide available as ab88230.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in human liver tissue lysate and the following whole cell lysates: HeLa; HepG2; Jurkat; HEK293; Ramos; SHSY5Y.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).

ターゲット情報

  • 機能
    Catalyzes the interconversion of N-acetylglucosamine to N-acetylmannosamine. Binds to renin forming a protein complex called high molecular weight (HMW) renin and inhibits renin activity.
  • 配列類似性
    Belongs to the N-acylglucosamine 2-epimerase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AGE antibody
    • GlcNAc 2 epimerase antibody
    • GlcNAc 2-epimerase antibody
    • N acetyl D glucosamine 2 epimerase antibody
    • N-acetyl-D-glucosamine 2-epimerase antibody
    • N-acylglucosamine 2-epimerase antibody
    • RBP antibody
    • RENBP antibody
    • RENBP_HUMAN antibody
    • Renin binding protein antibody
    • Renin-binding protein antibody
    • RnBP antibody
    see all

画像

  • All lanes : Anti-RENBP antibody (ab77081) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 6 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

プロトコール

参考文献

ab77081 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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