Anti-Rel B (phospho S573) 抗体 (ab47366)

製品の概要

  • 製品名Anti-Rel B (phospho S573) antibody
    Rel B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Rel B (phospho S573)
  • 特異性ab47366 detects endogenous levels of Rel B only when phosphorylated at serine 573.
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide corresponding to Human Rel B (phospho S573). Synthesized peptide derived from human RelB around the phosphorylation site of Ser573.
    Database link: Q01201

  • ポジティブ・コントロール
    • LoVo and RAW 264.7 cells, breast carcinoma tissue

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab47366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/20000.
WB 1/500 - 1/1000. Predicted molecular weight: 62 kDa.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49.
  • 配列類似性Contains 1 RHD (Rel-like) domain.
  • ドメインBoth N- and C-terminal domains are required for transcriptional activation.
  • 翻訳後修飾Phosphorylation at 'Thr-103' and 'Ser-573' is followed by proteasomal degradation.
  • 細胞内局在Nucleus. Cytoplasm > cytoskeleton > centrosome. Co-localizes with NEK6 in the centrosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • I REL antibody
    • I-Rel antibody
    • IREL antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
    • relB antibody
    • RELB_HUMAN antibody
    • Reticuloendotheliosis viral oncogene homolog B antibody
    • Transcription factor Rel B antibody
    • Transcription factor RelB antibody
    • v rel avian reticuloendotheliosis viral oncogene homolog B antibody
    • v rel reticuloendotheliosis viral oncogene homolog B antibody
    see all

Anti-Rel B (phospho S573) antibody 画像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47366. Image on the left is tissue untreated with immunising peptide; image on the right is tissue treated with immunising peptide.Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47366. Image on the left is tissue untreated with immunising peptide; image on the right is tissue treated with immunising peptide.
  • All lanes : Anti-Rel B (phospho S573) antibody (ab47366) at 1/500 dilution

    Lane 1 : LoVo (Human colorectal adenocarcinoma cell line) cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
    Lane 3 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate with phospho peptide


    Predicted band size : 62 kDa

Anti-Rel B (phospho S573) antibody (ab47366) 使用論文

ab47366 has not yet been referenced specifically in any publications.

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