製品の概要

  • 製品名Anti-Regucalcin antibody [17]
    Regucalcin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [17] to Regucalcin
  • アプリケーション適用あり: IHC-P, Flow Cyt, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human), expressed in E. coli.

  • ポジティブ・コントロール
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Proclin
    Constituents: Tris HCl, Sodium citrate, pH 7.0
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名17
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab67336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities.
  • 配列類似性Belongs to the SMP-30/CGR1 family.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Gluconolactonase antibody
    • GNL antibody
    • RC antibody
    • Regucalcin antibody
    • RGN antibody
    • RGN_HUMAN antibody
    • Senescence marker protein 30 antibody
    • SMP 30 antibody
    • SMP-30 antibody
    see all

Anti-Regucalcin antibody [17] 画像

  • Anti-Regucalcin antibody [17] (ab67336) at 1/1000 dilution + Human recombinant Regucalcin protein

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • IHC image of aRegucalcin staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab67336, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HepG2 cells stained with ab67336 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab67336, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Regucalcin antibody [17] (ab67336) 使用論文

ab67336 has not yet been referenced specifically in any publications.

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