製品の概要

  • 製品名Anti-REG4 antibody [MM0524-9B21]
    REG4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MM0524-9B21] to REG4
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human REG4 protein.

  • ポジティブ・コントロール
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human colon Adenocarcinoma.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)The IgG fraction of culture supernatant was purified by protein G affinity chromatography and 0.2 µm filtered.
  • ポリ/モノモノクローナル
  • クローン名MM0524-9B21
  • アイソタイプIgG2
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 18 kDa.

ターゲット情報

  • 機能May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium.
  • 組織特異性Highly expressed in the gastrointestinal tract including the duodenum, jejunum, ileum, ileocecum, appendix, descending colon, pancreas and small intestine. Weakly expressed in normal colon and stomach. Strongly expressed in most colorectal tumors than in normal colon. Preferentially expressed in mucinous tumors and in some cases neuro-endocrine tumors. Expressed in mucus-secreting cells and enterocyte-like cells. In small intestine expressed at the basal perinuclear zone of goblet cells.
  • 配列類似性Contains 1 C-type lectin domain.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Gastrointestinal secretory protein antibody
    • GISP antibody
    • Reg IV antibody
    • REG like protein antibody
    • REG-4 antibody
    • REG-like protein antibody
    • Reg4 antibody
    • REG4_HUMAN antibody
    • Regenerating gene type IV antibody
    • Regenerating islet derived family member 4 antibody
    • Regenerating islet derived protein 4 precursor antibody
    • Regenerating islet-derived protein 4 antibody
    • Regenerating islet-derived protein IV antibody
    • REGIV antibody
    • RELP antibody
    see all

Anti-REG4 antibody [MM0524-9B21] 画像

  • IHC image of REG4 staining in Human colon Adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89917, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-REG4 antibody [MM0524-9B21] (ab89917) 使用論文

ab89917 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"