Anti-Reelin 抗体 [G10] (ab78540)
Key features and details
- Mouse monoclonal [G10] to Reelin
- Suitable for: WB, IHC (PFA fixed)
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Reelin antibody [G10]
Reelin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [G10] to Reelin -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC (PFA fixed)more details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Fusion protein corresponding to Mouse Reelin aa 150-500.
Database link: Q60841 -
ポジティブ・コントロール
- Mouse liver or kidney. Rat brain lysate and Mouse brain membrane lysate. Mouse embryonic cerebral cortex.
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特記事項
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.242% Tris -
Concentration information loading...
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精製度
Tissue culture supernatant -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
G10 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab78540の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 388 kDa.
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IHC (PFA fixed) |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 388 kDa. |
IHC (PFA fixed)
Use at an assay dependent concentration. |
ターゲット情報
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機能
Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation. -
組織特異性
Abundantly produced during brain ontogenesis by the Cajal-Retzius cells and other pioneer neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In adult brain, preferentially expressed in GABAergic interneurons of prefrontal cortices, temporal cortex, hippocampus and glutamatergic granule cells of cerebellum. Expression is reduced to about 50% in patients with schizophrenia. Also expressed in fetal and adult liver. -
関連疾患
Lissencephaly 2 -
配列類似性
Belongs to the reelin family.
Contains 16 BNR repeats.
Contains 8 EGF-like domains.
Contains 1 reelin domain. -
発生段階
Expressed in fetal and postnatal brain and liver. Expression in postnatal human brain is high in the cerebellum. -
ドメイン
The basic C-terminal region is essential for secretion. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 5649 Human
- Entrez Gene: 19699 Mouse
- Entrez Gene: 24718 Rat
- Omim: 600514 Human
- SwissProt: P78509 Human
- SwissProt: Q60841 Mouse
- SwissProt: P58751 Rat
- Unigene: 655654 Human
see all -
別名
- LIS2 antibody
- PRO1598 antibody
- Reeler antibody
see all
画像
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ab78540, at 1/1000 dilution staining Reelin in embryonic cerebral cortex at 15 days in utero, with the pial surface above and the ventricle below. The positive zone is the Cajal-Retzius cell layer in the marginal zone.
This image was generated using the ascites version of the product.
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All lanes : Anti-Reelin antibody [G10] (ab78540) at 1/500 dilution
Lane 1 : Rat brain lysate
Lane 2 : Mouse brain membrane lysate
Secondary
All lanes : Goat anti-mouse secondary antibody
conjugated to HRP
Predicted band size: 388 kDa
Observed band size: 388 kDa
Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.This image was generated using the ascites version of the product.
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Immunolocalization of Reelin (red) in the cortex of e14.5 mouse CNS using ab78540 at 1/1000 dilution. The blue stain is Hoechst nuclear counter stain.
This image was generated using the ascites version of the product.
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Anti-Reelin antibody [G10] (ab78540) at 1/500 dilution + Rat brain lysate at 10 µg
Secondary
Goat anti-mouse secondary
antibody conjugated to HRP
Predicted band size: 388 kDa
Observed band size: 388 kDaThis image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (30)
ab78540 は 30 報の論文で使用されています。
- Ardalan M et al. Reelin cells and sex-dependent synaptopathology in autism following postnatal immune activation. Br J Pharmacol 179:4400-4422 (2022). PubMed: 35474185
- Mercier MS et al. Long-term potentiation in neurogliaform interneurons modulates excitation-inhibition balance in the temporoammonic pathway. J Physiol 600:4001-4017 (2022). PubMed: 35876215
- Caramello A et al. Dentate gyrus development requires a cortical hem-derived astrocytic scaffold. Elife 10:N/A (2021). PubMed: 33393905
- Gavrilovici C et al. Behavioral Deficits in Mice with Postnatal Disruption of Ndel1 in Forebrain Excitatory Neurons: Implications for Epilepsy and Neuropsychiatric Disorders. Cereb Cortex Commun 2:tgaa096 (2021). PubMed: 33615226
- Kang ZC et al. Pinocembrin Ameliorates Cognitive Impairment Induced by Vascular Dementia: Contribution of Reelin-dab1 Signaling Pathway. Drug Des Devel Ther 14:3577-3587 (2020). PubMed: 32943845