製品の概要

  • 製品名
    Anti-Reelin antibody [G10]
    Reelin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [G10] to Reelin
  • アプリケーション
    適用あり: WB, IHC (PFA fixed), ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant fusion protein, corresponding to amino acids 164-496 of Mouse Reelin

  • ポジティブ・コントロール
    • Mouse liver or kidney. Rat brain lysate and Mouse brain membrane lysate. Mouse embryonic cerebral cortex.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 388 kDa.
IHC (PFA fixed) 1/1000 - 1/2000.
ICC Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation.
  • 組織特異性
    Abundantly produced during brain ontogenesis by the Cajal-Retzius cells and other pioneer neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In adult brain, preferentially expressed in GABAergic interneurons of prefrontal cortices, temporal cortex, hippocampus and glutamatergic granule cells of cerebellum. Expression is reduced to about 50% in patients with schizophrenia. Also expressed in fetal and adult liver.
  • 関連疾患
    Lissencephaly 2
  • 配列類似性
    Belongs to the reelin family.
    Contains 16 BNR repeats.
    Contains 8 EGF-like domains.
    Contains 1 reelin domain.
  • 発生段階
    Expressed in fetal and postnatal brain and liver. Expression in postnatal human brain is high in the cerebellum.
  • ドメイン
    The basic C-terminal region is essential for secretion.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • LIS2 antibody
    • PRO1598 antibody
    • Reeler antibody
    • Reelin antibody
    • RELN antibody
    • RELN_HUMAN antibody
    • RL antibody
    see all

画像

  • ab78540, at 1/1000 dilution staining Reelin in embryonic cerebral cortex at 15 days in utero, with the pial surface above and the ventricle below. The positive zone is the Cajal-Retzius cell layer in the marginal zone.
  • All lanes : Anti-Reelin antibody [G10] (ab78540) at 1/500 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Mouse brain membrane lysate

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size : 388 kDa
    Observed band size : 388 kDa
    Additional bands at : 140 kDa. We are unsure as to the identity of these extra bands.
  • Immunolocalization of Reelin (red) in the cortex of e14.5 mouse CNS using ab78540 at 1/1000 dilution. The blue stain is Hoechst nuclear counter stain.
  • Anti-Reelin antibody [G10] (ab78540) at 1/500 dilution + Rat brain lysate at 10 µg

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size : 388 kDa
    Observed band size : 388 kDa

参考文献

This product has been referenced in:
  • Frazer S  et al. Transcriptomic and anatomic parcellation of 5-HT3AR expressing cortical interneuron subtypes revealed by single-cell RNA sequencing. Nat Commun 8:14219 (2017). IHC . Read more (PubMed: 28134272) »
  • Kaneko Y  et al. Kainic Acid-Induced Golgi Complex Fragmentation/Dispersal Shifts the Proteolysis of Reelin in Primary Rat Neuronal Cells: An In Vitro Model of Early Stage Epilepsy. Mol Neurobiol 53:1874-1883 (2016). ICC . Read more (PubMed: 25790952) »

See all 8 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: RT°C
Sample
Mouse Cultured Cells (Primary cortical cells)
Specification
Primary cortical cells
Permeabilization
Yes - Acetone - 10 minutes
Fixative
Paraformaldehyde
Username

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Verified customer

投稿 Jul 24 2013

Merci de nous avoir contactés.

Comme convenu j'ai mis en place l'envoi d'une unité de ab18570 en remplacement du ab78540, le numéro de commande de remplacement gratuit de ce produit est ***.
Vous recevrez prochainement...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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