製品の概要
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製品名Anti-RECQL5 antibody
RECQL5 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to RECQL5
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由来種Rabbit
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アプリケーション適用あり: IHC-P, WB, IPmore details
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種交差性交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region within N terminal amino acids 1 - 50 (MSSHHTTFPFDPERRVRSTLKKVFGFDSFKTPLQESATMAVVKGNKDVF V) of Human RECQL5 (NP_004250.4).
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ポジティブ･コントロール
- HeLa cell lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7.0-8.0 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab91422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 109 kDa. | |
| IP | Use at 5-15 µg/mg of lysate. |
ターゲット情報
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機能May have an important role in DNA metabolism.
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組織特異性Ubiquitous.
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配列類似性Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
細胞内局在Cytoplasm and Nucleus > nucleoplasm.
- Information by UniProt
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参照データベース
- Entrez Gene: 101137561 Gorilla
- Entrez Gene: 9400 Human
- Omim: 603781 Human
- SwissProt: O94762 Human
- Unigene: 632229 Human
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別名
- ATP-dependent DNA helicase Q5 antibody
- DNA helicase antibody
- DNA helicase, RecQ like type 5 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQL5 antibody (ab91422)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling RECQL5 with ab91422 at 1/1000 (1µg/ml). Detection: DAB. -
Western blot - Anti-RECQL5 antibody (ab91422)All lanes : Anti-RECQL5 antibody (ab91422) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Predicted band size: 109 kDa -
Immunoprecipitation - Anti-RECQL5 antibody (ab91422)SSA1 was immunoprecipitated from 1mg HeLa whole cell lysate using 10µg ab91422.
20% of the immunoprecipitate was loaded per lane, and probed with ab91422 at 1µg/ml (lane 1) or a control IgG (lane2).
Detection: chemoluminescence with an exposure time of 10 seconds.
参考文献
This product has been referenced in:
- Patterson K et al. Altered RECQL5 expression in urothelial bladder carcinoma increases cellular proliferation and makes RECQL5 helicase activity a novel target for chemotherapy. Oncotarget 7:76140-76150 (2016). WB ; Human . Read more (PubMed: 27764811) »
- Kantidakis T et al. Mutation of cancer driver MLL2 results in transcription stress and genome instability. Genes Dev 30:408-20 (2016). Read more (PubMed: 26883360) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"