All lanes : Anti-RECQL5 antibody (ab91419) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg
Developed using the ECL technique
Predicted band size : 109 kDa
Exposure time : 3 minutes
Immunoprecipitation - RECQL5 antibody (ab91419)
SSA1 was immunoprecipitated from 1mg HeLa whole cell lysate using 10µg ab91419. 20% of the immunoprecipitate was loaded per lane, and probed with ab91419 at 1µg/ml (lane 1) or a control IgG (lane2). Detection: chemoluminescence with an exposure time of 10 seconds.