Recombinant X. laevis PKC alpha protein (ab60839)

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製品の概要

法規制情報

カルタヘナ法

製品の詳細

  • 由来
    Recombinant
  • 由来
    Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種
      Xenopus laevis

関連製品

特性

Our Abpromise guarantee covers the use of ab60839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 製品の状態
    Liquid
  • 備考

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • AAG6
    • Aging associated gene 6
    • aPKC
    • KPCA_HUMAN
    • PKC alpha
    • PKC-A
    • PKC-alpha
    • PKCA
    • PRKACA
    • PRKCA
    • Protein Kinase C alpha
    • Protein kinase C alpha type
    see all
  • 機能
    This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • 配列類似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 細胞内局在
    Cytoplasm. Cell membrane. Nucleus.
  • Information by UniProt

画像

  • Functional Studies - PKC alpha protein (Tagged) (ab60839)
    Functional Studies - PKC alpha protein (Tagged) (ab60839)
    Sample Kinase Activity Plot.
  • SDS-PAGE - PKC alpha protein (Tagged) (ab60839)
    SDS-PAGE - PKC alpha protein (Tagged) (ab60839)
    ab60839 on SDS-PAGE, MW ~103 kDa.

参考文献

ab60839 has not yet been referenced specifically in any publications.

ab60839 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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I ordered PKC alpha protein (Active).(ab60839, Serial no: k1111-900) from your company, and want to use it to phosphorylate an E.Coli purified protein, but I could not find the protocol or the recipe of kinase reaction buffer for this kinase on your we...

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Thank you for contacting us.

Please find attached a modified version of the radiolabel (phosphorus 33-ATP) protocol that was used to measure activity of ab60839. The assay utilized CREBtide as the substrate (KRREILSRRPSYR) but the lab believ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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