製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • アクセッション番号P01135
    • 生物種Human
    • 配列VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA
    • 分子量17 kDa
    • 領域40 to 89

関連製品

特性

Our Abpromise guarantee covers the use of ab9587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 製品の状態Lyophilised
  • 備考The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is < 0.2 ng/ml, corresponding to a specific activity of > 5 x 106 units/mg.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成Reconstitute with 100ul of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.

関連情報

  • 別名
    • EGF like TGF
    • EGF-like TGF
    • ETGF
    • TFGA
    • TGF A
    • TGF type 1
    • TGF-alpha
    • Tgfa
    • TGFA_HUMAN
    • Transforming growth factor alpha
    • Transforming growth factor alpha precursor
    • Wa1
    • Waved 1
    see all
  • 機能TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
  • 組織特異性Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.
  • 配列類似性Contains 1 EGF-like domain.
  • 細胞内局在Cell membrane and Secreted > extracellular space.
  • Information by UniProt

Recombinant TGF alpha protein (ab9587) 使用論文

This product has been referenced in:
  • Hobor S  et al. TGFa and amphiregulin paracrine network promotes resistance to EGFR blockade in colorectal cancer cells. Clin Cancer Res 20:6429-38 (2014). Read more (PubMed: 24916700) »

See 1 Publication for this product

Product Wall

Thank you once again for your enquiry. I am pleased to forward you some futher procedure details for testing of ab in cell culture. I hope this will be helpful to you. 1. Resuspend 3T3 cells (passage#38) in regular culture media with 10% calf...

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Thank you for your enquiry and thank you very much for your patience. I do apologize for the delay, it some time to get this information from the originator of these peptides. For each of the products listed, included is the concentration of each pr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"