製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • 配列
      MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ
    • 分子量
      17 kDa including tags
    • 領域
      25 to 183
    • タグ
      His tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab78833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 精製度
    >95% by SDS-PAGE .

  • 製品の状態
    Liquid
  • 備考

    Concentration is determined by BCA assay

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20mM Tris, pH 7.5

関連情報

  • 別名
    • SA V1
    • SA V2
    • strepavidin
    • Streptavidin V1
    • Streptavidin V2
    see all
  • 関連性
    Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
  • 細胞内局在
    Cytoplasmic

画像

  • 15% SDS-PAGE showing ab78833 at approximately 17kDa (3µg).

参考文献

This product has been referenced in:
  • Qin GW et al. Surface modification of permalloy (Ni80Fe20) nanoparticles for biomedical applications Journal of Nanoparticle Research 13:45-51 (2010). ELISA . Read more (PubMed: ) »

See 1 Publication for this product

レビューと Q&A

We weren’t sure what your client means by “accessible”. If they’re asking whether it’s detectable with a His tag antibody, then in principle it should be. If they are asking whether it’s the cleavable form, regrettab...

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I can confirm that the His tag is functional in this peptide for complexing with Nickel. The Met and Val in front of the His tag helps the His tag for proper configuration and has no impact on the protein’s functionality. I hope this information is...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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