Recombinant staphylococcus aureus Glutamyl endopeptidase protein (ab98127)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: HPLC, SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant staphylococcus aureus Glutamyl endopeptidase protein -
生理活性
ab98127 cleaves at the Carboxyl side of E (can also cleave D under certain conditions). Reaction Buffer: 10 mM Tris, pH 8.0 -
精製度
> 95 % SDS-PAGE.
ab98127 is sterile filtered through a 0.2 micron filter. Purity is > 95% by SDS-PAGE gel and HPLC analyses. -
エンドトキシン・レベル
< 0.100 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Staphylococcus aureus -
配列
LPNNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKHV VDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSPN EQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKITY LKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENV RNFLKQNIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPD NPDNGDNNNSDNPDAA -
予測される分子量
29 kDa -
領域
71 to 336
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特性
Our Abpromise guarantee covers the use of ab98127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
HPLC
SDS-PAGE
Functional Studies
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製品の状態
Lyophilized -
備考
ab98127 cleaves at the Carboxyl side of E (can also cleave D under certain conditions). Reaction Buffer: 10 mM Tris, pH 8.0 ab98127 cleaves at the Carboxyl side of E (can also cleave D under certain conditions). Reaction Buffer: 10 mM Tris, pH 8.0 -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituent: 0.121% Tris
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成The lyophilized protein is stable at room temperature for up to 1 month. Reconstitute in water to a concentration of 0.1-1.0 mg/ml.
関連情報
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別名
- Endoproteinase Glu C
- sspA
- Staphylococcal serine proteinase
see all -
関連性
Glutamyl endopeptidase preferentially cleaves peptide bonds on the carboxyl-terminal side of aspartate and glutamate. Along with other extracellular proteases it is involved in colonization and infection of human tissues. It is required for proteolytic maturation of thiol protease sspB and inactivation of sspC, an inhibitor of sspB. It is the most important protease for degradation of fibronectin-binding protein (FnBP) and surface protein A, which are involved in adherence to host cells. It may also protect bacteria against host defense mechanism by cleaving the immunoglobulin classes IgG, IgA and IgM. It may be involved in the stability of secreted lipases.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab98127 は論文での使用が確認できていません。