Recombinant Soluble TNF Receptor I protein (ab9980)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli

関連製品

特性

Our Abpromise guarantee covers the use of ab9980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • エンドトキシン・レベル< 0.100 Eu/µg
  • 製品の状態Lyophilised
  • 備考The biological activity of this product was determined by its inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L929 cells. The ED50 for this effect, in the presence of 0.25 ng/ml of recombinant human TNF-alpha is 0.05 µg/ml.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成Please reconstitute this product in 200ul water.

関連情報

  • 別名
    • CD120a
    • TBP1
    • TNFR1
    • TNFR55
    • TNFRSF1A
    • Tumor necrosis factor receptor 1
    see all
  • 関連性TNF RI (CD120a) is a 55 kDa transmembrane glycoprotein that is expressed by virtually all nucleated mammalian cells. Among the numerous cells known to express TNF RI are hepatocytes, monocytes and neutrophils, cardiac muscle cells, endothelial cells, and CD34+ hematopoietic progenitors. Both TNF alpha and TNF beta bind to TNF RI. Soluble TNF alpha binds with a Kda in the range of 20-60 pM, while TNF beta binds with a Kda equal to 650 pM. TNF RI relative to TNF RII seems to be the more physiologically relevant receptor, whereas TNF R2 appears to play a direct role in only a limited number of TNF responses. Soluble TNF RI, which blocks TNF alpha activity, has been identified in both urine and blood (1-3 ng/mL). Serum levels of sTNF receptors increase dramatically in certain pathological situations.
  • 細胞内局在Cytoplasmic

Recombinant Soluble TNF Receptor I protein (ab9980) 使用論文

ab9980 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Please see the reconstitution protocol below: Centrifuge the vial prior to opening. Reconstitute in 5 mM Tris pH 8.0 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and...

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Thank you for your enquiry. This antibody has been lyophilised from 5mM Tris, pH 8.0 and should be reconstituted in dH2O to the desired concentration. I hope this information helps, please do not hesitate to contact us if you need any more advic...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"