Recombinant Simian Virus 40 (SV40) Simian Virus 40 Major Capsid VP1 protein (ab74565)

製品の概要

  • 製品名Recombinant Simian Virus 40 (SV40) Simian Virus 40 Major Capsid VP1 protein
  • タンパク質長Full length protein

製品の詳細

  • 由来Recombinant
  • 由来Saccharomyces cerevisiae
  • アミノ酸配列
    • 生物種Simian Virus 40 (SV40)

特性

Our Abpromise guarantee covers the use of ab74565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    ELISA

    Western blot

    SDS-PAGE

  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C.

    Preservative: None
    Constituents: PBS

  • 再構成Reconstitute with deionized H2O.

関連情報

  • 別名
    • Major capsid protein VP1
    • Major structural protein VP1
  • 関連性Simian virus 40 (SV40) is a small, non enveloped DNA virus with an icosahedral capsid of 45 nm. The simian virus 40 capsid is composed of 72 pentamers of VP1, the major protein of SV40. These pentamers are arranged in a T=7d icosahedral surface lattice, which is maintained by three types of appropriately arranged, non-equivalent interactions between the pentamers. Simian Virus 40 Major Capsid VP1 binds to N-glycolylneuraminic analog of the ganglioside GM1 on the cell surface to provide virion attachment to target cell. Once attached, the virion enters a caveolae and traffics to the endoplasmic reticulum. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. The assembly takes place in the cell nucleus, encapsulates the genomic DNA and participates in rearranging nucleosomes around the viral DNA. The viral progenies exit the cells by lytic release.
  • 細胞内局在Virion. Nucleus. Endoplasmic reticulum. Note=Following host cell entry, the virion enters into the endoplasmic reticulum through a calveolar-dependent pathway. Then, viral DNA is translocated to the nucleus. Shortly after synthesis, a nuclear localization signal directs VP1 to the cell nucleus where virion assembly occurs.

Recombinant Simian Virus 40 (SV40) Simian Virus 40 Major Capsid VP1 protein 画像

  • SDS-PAGE showing ab74565 at approximately 44kDa (4.2µg/lane)

Recombinant Simian Virus 40 (SV40) Simian Virus 40 Major Capsid VP1 protein (ab74565) 使用論文

ab74565 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply. I can confirm that the antibody ab34756 will react with the SV40 VP1 protein ab74565. On the antibody datasheet in the section labelled "cross-reactivity", it notes that it does react with the VP1 protein of SV40. I hope this...

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Thank you for your call this week and for your patience while I have been in touch with the production lab about these proteins. I have confirmed with the lab that these proteins do not contain any tag. To detect them, you could use specific monoclonal...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"