The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 1 x 107 IU/mg.
% SDS-PAGE. ab73378 was purified by proprietary chromatographic techniques.
Purity is greater than 95.0% as determined by both RP-HPLC and SDS-PAGE.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: PBS, pH 7.4
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitute in sterile 18MOhm/cm H2O to not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Interferon epsilon 1
Interferon tau 1
Expressed in prostate cancer cell line PC-3, amnion-derived WISH cells, melanoma cell line SK-MEL-28, cell line Daudi and very weakly in MCF-7 human breast cancer cells. Not detected in cell lines Namalwa (Burkitt's lymphoma) and Jurkat (T-cell).