Recombinant S. cerevisiae KEX2 protease protein (ab96554)

製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected BTI-TN-5B1-4 cells
  • アミノ酸配列
    • アクセッション番号P13134
    • 生物種Saccharomyces cerevisiae
    • 配列APPMDSSLLP VKEAEDKLSI NDPLFERQWH LVNPSFPGSD INVLDLWYNN ITGAGVVAAI VDDGLDYENE DLKDNFCAEG SWDFNDNTNL PKPRLSDDYH GTRCAGEIAA KKGNNFCGVG VGYNAKISGI RILSGDITTE DEAASLIYGL DVNDIYSCSW GPADDGRHLQ GPSDLVKKAL VKGVTEGRDS KGAIYVFASG NGGTRGDNCN YDGYTNSIYS ITIGAIDHKD LHPPYSEGCS AVMAVTYSSG SGEYIHSSDI NGRCSNSHGG TSAAAPLAAG VYTLLLEANP NLTWRDVQYL SILSAVGLEK NADGDWRDSA MGKKYSHRYG FGKIDAHKLI EMSKTWENVN AQTWFYLPTL YVSQSTNSTE ETLESVITIS EKSLQDANFK RIEHVTVTVD IDTEIRGTTT VDLISPAGII SNLGVVRPRD VSSEGFKDWT FMSVAHWGEN GVGDWKIKVK TTENGHRIDF HSWRLKLFGE SIDSSKTETF VFGNDKEEVE PAATESTVSQ YSASSTSISI SATSTSSISI GVETSAIPQT TTASTDPDSD PNTP
    • 分子量60 kDa
    • 領域114 to 667

特性

Our Abpromise guarantee covers the use of ab96554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性ab96554 from High-5 insect cells contains the same specific activity and recognition sequence specificity as yeast derived KEX2. 1 milligram of recombinant KEX2 contains activity equivalent to at least 40 units of yeast derived KEX2. Cleaves at the carboxyl side of K/R-R.
  • アプリケーション

    SDS-PAGE

    Functional Studies

  • エンドトキシン・レベル< 0.100 Eu/µg
  • 精製度> 95 % SDS-PAGE.
    Purity is greater than 95% by SDS-PAGE gel and HPLC analyses.
  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 5mM Calcium chloride, 10mM Sodium acetate, pH 6.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成The lyophilized protein is stable at room temperature for up to 1 month. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.

関連情報

  • 別名
    • KEX 2
    • Kex2p
    • Kexin KEX2
    • QDS1
    • SRB1
    • VMA45
    see all
  • 関連性Kex2 in the yeast Saccharomyces cerevisiae is a transmembrane, Ca2+-dependent serine protease of the subtilisin-like pro-protein convertase (SPC) family with specificity for cleavage after paired basic amino acids. At steady state, Kex2 is predominantly localized in late Golgi compartments and initiates the proteolytic maturation of pro-protein precursors that transit the distal secretory pathway. However, Kex2 localization is not static, and its itinerary apparently involves transiting out of the late Golgi and cycling back from post-Golgi endosomal compartments during its lifetime.
  • 細胞内局在Golgi apparatus; trans-Golgi network; trans-Golgi network membrane; single-pass type I membrane protein.

Recombinant S. cerevisiae KEX2 protease protein (ab96554) 使用論文

ab96554 has not yet been referenced specifically in any publications.

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The KEX2 protease protein (ab96554)is formulated using 10 mM sodiumacetate, pH6.0 +5 mMcalcium chloride and then lyophilised from this buffer. This is the product you receive. The sodium acetate and calcium chlori...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"