Recombinant S. cerevisiae GAL4 protein (ab81879)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant S. cerevisiae GAL4 protein
GAL4 タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE.
ab81879 is isolated from an E. coli strain that carries the coding sequence of the fused protein under the control of a T7 promoter. -
発現系
Escherichia coli -
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Saccharomyces cerevisiae -
領域
1 to 147
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製品の詳細
Recombinant S. cerevisiae GAL4 protein
特性
Our Abpromise guarantee covers the use of ab81879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
Transcriptional activity is greatly stimulated by promoter-specific activator proteins. These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain. The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5`-CGGN5(T/A)N5CCG-3`. A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. Linking of an acidic synthetic peptide, forming an {alpha}-helix, to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure. This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.9
Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)
関連情報
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別名
- Antigen NY CO 27
- Antigen NY-CO-27
- Antigen NYCO27
see all -
機能
Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions. -
配列類似性
Contains 2 galectin domains. -
ドメイン
Contains two homologous but distinct carbohydrate-binding domains. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab81879 は論文での使用が確認できていません。