Recombinant S. cerevisiae GAL4 protein (ab81879)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Saccharomyces cerevisiae
    • 領域1 to 147

特性

Our Abpromise guarantee covers the use of ab81879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 精製度> 95 % SDS-PAGE.
    ab81879 is isolated from an E. coli strain that carries the coding sequence of the fused protein under the control of a T7 promoter.
  • 製品の状態Liquid
  • 備考


    Transcriptional activity is greatly stimulated by promoter-specific activator proteins. These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain. The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5`-CGGN5(T/A)N5CCG-3`. A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. Linking of an acidic synthetic peptide, forming an {alpha}-helix, to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure. This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

関連情報

  • 別名
    • Antigen NY CO 27
    • Antigen NY-CO-27
    • Antigen NYCO27
    • GAL 4
    • Gal-4
    • GAL4
    • Galectin 4
    • Galectin-4
    • Galectin4
    • Homo sapiens galectin4 mRNA complete cds
    • L 36 lactose binding protein
    • L-36 lactose-binding protein
    • L36 lactose binding protein
    • L36LBP
    • Lactose binding lectin 4
    • Lactose-binding lectin 4
    • Lectin galactoside binding soluble 4
    • LEG4_HUMAN
    • LGALS4
    see all
  • 機能Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
  • 配列類似性Contains 2 galectin domains.
  • ドメインContains two homologous but distinct carbohydrate-binding domains.
  • Information by UniProt

Recombinant S. cerevisiae GAL4 protein (ab81879) 使用論文

ab81879 has not yet been referenced specifically in any publications.

Product Wall

The GAL4 –AH (P1018) has a fragment 1-147 aa and an additiona alpha helix sequence (denoted AH).

Please find the answer from the lab and the attached data:

"The protein contains no tag but an alpha-helix peptide on its C-terminus. Yes, the protein is active for gel shift experiment. Attached please find the gel shift experimental dat...

Read More

Thank you for contacting us.

In the absence of the alpha helix, the GAL4 protein willbind to DNA but will not activate transcription. The alpha helix allows the protein complex to activate genes involved in galactose metabolism.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"