Recombinant S. cerevisiae GAL4 protein (ab81879)

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製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • 生物種
      Saccharomyces cerevisiae
    • 領域
      1 to 147

特性

Our Abpromise guarantee covers the use of ab81879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 精製度
    > 95 % SDS-PAGE.
    ab81879 is isolated from an E. coli strain that carries the coding sequence of the fused protein under the control of a T7 promoter.
  • 製品の状態
    Liquid
  • 備考


    Transcriptional activity is greatly stimulated by promoter-specific activator proteins. These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain. The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5`-CGGN5(T/A)N5CCG-3`. A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. Linking of an acidic synthetic peptide, forming an {alpha}-helix, to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure. This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

関連情報

  • 別名
    • Antigen NY CO 27
    • Antigen NY-CO-27
    • Antigen NYCO27
    • GAL 4
    • Gal-4
    • GAL4
    • Galectin 4
    • Galectin-4
    • Galectin4
    • Homo sapiens galectin4 mRNA complete cds
    • L 36 lactose binding protein
    • L-36 lactose-binding protein
    • L36 lactose binding protein
    • L36LBP
    • Lactose binding lectin 4
    • Lactose-binding lectin 4
    • Lectin galactoside binding soluble 4
    • LEG4_HUMAN
    • LGALS4
    see all
  • 機能
    Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
  • 配列類似性
    Contains 2 galectin domains.
  • ドメイン
    Contains two homologous but distinct carbohydrate-binding domains.
  • Information by UniProt

参考文献

ab81879 has not yet been referenced specifically in any publications.

ab81879 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

3 reviews or Q&A

10
Votes: Highest First

BTW, just to be sure - in the figure that they sent us, the protein that we are talking about is gal4-147? What is the gal4-AH protein?

The GAL4 –AH (P1018) has a fragment 1-147 aa and an additiona alpha helix sequence (denoted AH).

Here are a few questions regarding ab81879:

We have few questions regarding this product that will enable us to decide if we order the product :

1. Does the gal4 protein contain any tag? If so, where is it fused (N or C terminal parts...

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Please find the answer from the lab and the attached data:

"The protein contains no tag but an alpha-helix peptide on its C-terminus. Yes, the protein is active for gel shift experiment. Attached please find the gel shift experimental dat...

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What is the purpose of the alpha helix on thisGAL4 protein?

Thank you for contacting us.

In the absence of the alpha helix, the GAL4 protein willbind to DNA but will not activate transcription. The alpha helix allows the protein complex to activate genes involved in galactose metabolism.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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