製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • 分子量
      28 kDa
    • 配列の追加情報
      The protein is a 27.6 kDa monomer containing a N-terminus 6x His-Tag.

特性

Our Abpromise guarantee covers the use of ab51993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Western blot

    Flow Cytometry

  • 精製度
    > 95 % SDS-PAGE.
    The recombinant RFP (Red Fluorescence Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal fluorescence of the protein.
    Endotoxin was removed for in vivo injection or cell culture applications. Endotoxin level equal to or less than 0.1 ng/µg.
  • 製品の状態
    Lyophilised
  • 備考

    This protein (DSRed) is suitable as control reagent for RFP expression studies or as a labeling reagent.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

  • 再構成
    Reconstitute with 100 µl dH2O to 1mg/ml

関連情報

  • 別名
    • DsRed
    • GFP like chromoprotein
    • red fluorescent protein
    • RFP
  • 関連性
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Recombinant RFP protein 画像

  • ab51993 loaded under reducing conditions and stained with coomassive blue.

Recombinant RFP protein (ab51993) 使用論文

This product has been referenced in:
  • Dufour YS  et al. Direct Correlation between Motile Behavior and Protein Abundance in Single Cells. PLoS Comput Biol 12:e1005041 (2016). Read more (PubMed: 27599206) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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