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Our Abpromise guarantee covers the use of ab59110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific Activity: >300 units/mg protein. The activity of recombinant rat nNOS (neuronal) is determined by an oxyhemoglobin assay that measures the reaction of nitric oxide with oxyhemoglobin to yield methemoglobin. One unit of enzyme produces 1 nmole of nitric oxide per minute at 37°C in 50 mM HEPES, pH 7.4, containing 5µM oxyhemoglobin, 1 mM CaCl2, 20µg/ml calmodulin, 0.1 mM NADPH, 50µM arginine, 12µM tetrahydrobiopterin, and 170µM DTT. nNOS (neuronal) is calcium/ calmodulin dependent and has a Km for arginine of approximately 2µM.
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 1µM Tetrahydrobiopterin, 20% Glycerol, 50mM HEPES, 100mM Sodium chloride, pH 7.4
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ab59110 has not yet been referenced specifically in any publications.