Recombinant rat Interferon gamma protein (ab645)

製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Rat
    • 配列MQGTLIESLESLKNYFNSSSMDAMEGKSLLLDIWRNWQKDGNTKILESQI ISFYLRLFEVLKDNQAISNNISVIESHLITNFFSNSKAKKDAFMSIAKFE VNNPQIQHKAVNELIRVIHQLSPESSLRKRKRSRC
    • 分子量16 kDa

特性

Our Abpromise guarantee covers the use of ab645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • エンドトキシン・レベル< 0.100 Eu/µg
  • 製品の状態Lyophilised
  • 備考The ED50, determined by a cytopathic effect inhibition assay with murine L929 cells challenged with EMC virus is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Stable for 12 months at -20°C.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成Reconstitute with 10 mM sodium phosphate (pH 8.0) to a concentration of 0.1 - 1 mg/mL.

関連情報

  • 別名
    • IFG
    • IFI
    • IFN gamma
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    see all
  • 機能Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • 組織特異性Released primarily from activated T lymphocytes.
  • 関連疾患In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • 配列類似性Belongs to the type II (or gamma) interferon family.
  • 翻訳後修飾Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • 細胞内局在Secreted.
  • Information by UniProt

Recombinant rat Interferon gamma protein (ab645) 使用論文

This product has been referenced in:
  • Johnstone SA  et al. Comparison of human olfactory and skeletal MSCs using osteogenic nanotopography to demonstrate bone-specific bioactivity of the surfaces. Acta Biomater 13:266-76 (2015). Read more (PubMed: 25463488) »
  • Hall ME  et al. Rescue of cardiac leptin receptors in db/db mice prevents myocardial triglyceride accumulation. Am J Physiol Endocrinol Metab 307:E316-25 (2014). Read more (PubMed: 24939734) »

See all 2 Publications for this product

Product Wall

Dear Dr. Titheradge, Thank you for your patience. The rat IFN-gamma (ab645) was successfully tested in murine L929 cells - ab645 is cross reactive with mouse cells. We have not tested the mouse IFN-gamma in rat cells and do not know if ab9922 ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"