Recombinant rat IL-10 protein (Active) (ab9970)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/g
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
-
製品名
Recombinant rat IL-10 protein (Active)
IL-10 タンパク質・ペプチド 製品一覧 -
生理活性
Determined by the dose-dependent inhibition of antigen-specific T-cell proliferation. Dose is lot dependent.
-
精製度
> 98 % SDS-PAGE.
Sterile filtered. Greater than 98% pure by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g). Lot dependent. -
エンドトキシン・レベル
< 1.000 Eu/g -
発現系
Escherichia coli -
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
生物種
Rat -
配列
Interleukin-10, originally known as Cytokine Synthesis Inhibitory factor (CSIF), is an 18.7 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI: SKGHSIKGDN NCTHFPVSQT HMLRELRAAF SQVKTFFQKK DQLDNIVLTD SLLQDFKGYL GCQALSEMIK FYLVEVMPQA ENHGPEIKEH LNSLGEKLKT LWIQLRRCHR FLPCENKSKA VEQVKNDFNK LQDKGVYKAM NEFDIFINCI EAYVTLKMKN -
領域
19 to 178
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab9970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Functional Studies
SDS-PAGE
-
製品の状態
Lyophilized -
備考
The ED50, determined by the dose-dependent inhibition of antigen-specific T cell proliferation, is < 10 ng/ml -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 0.328% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
再構成For lot specific reconstitution information please contact our Scientific Support Team.
関連情報
-
別名
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all -
機能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
組織特異性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
配列類似性
Belongs to the IL-10 family. -
細胞内局在
Secreted. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab9970 は論文での使用が確認できていません。