Recombinant Rat G protein alpha (mutated L227 + N295) (ab90406)

製品の概要

法規制情報

製品の詳細

  • 由来Recombinant
  • 由来Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種Rat

特性

Our Abpromise guarantee covers the use of ab90406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

  • 製品の状態Liquid
  • 備考


    GTP-binding possesses a highly conserved aspartate residue in the NKXD motif that is critical for high-affinity interaction with GTP. In almost all GTP-binding proteins so far, the D/N-mutation switches base-specifity from guanine to xanthine. Whereas the exchange of Asp295 to Asn295 leads to inactive mutants of Galpha-subunits, an additional Q/L-mutation in the catalytic site (Gln227 to Leu227) rescues protein function and induces xanthine nucleotide-specifity.

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前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 75mM Tris HCl, 12.5mM Magnesium chloride, 1mM EDTA, pH 7.4

関連情報

  • 別名
    • Adenylate cyclase stimulating G alpha protein
    • AHO
    • C20orf45
    • Extra large alphas protein
    • GNAS complex locus
    • GNAS1
    • GPSA
    • GSA
    • GSP
    • Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1
    • Guanine nucleotide binding protein G(s) alpha subunit
    • Guanine nucleotide binding protein G(s) subunit alpha isoforms XLas
    • guanine nucleotide regulatory protein
    • NESP
    • Neuroendocrine secretory protein
    • PHP1A
    • PHP1B
    • PHP1C
    • POH
    • RP4-543J19.4
    • Secretogranin VI
    • XLalphas
    see all
  • 関連性GTP-binding proteins (G-proteins)are a family of heterotrimeric proteins that play a critical role in signal transduction by coupling cell surface, 7-transmembrane domain receptors to intracellular signaling pathways including second messenger generation (such as cyclic AMP, calcium and diacylglycerol), protein phosphorylation, ion channel activation, gene induction, cell growth and differentiation. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit.
  • 細胞内局在Cell Membrane

Recombinant Rat G protein alpha (mutated L227 + N295) (ab90406) 使用論文

ab90406 has not yet been referenced specifically in any publications.

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