製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Rat
    • 配列
      NSNTGCPPSYDGYCLNGGVCMYVESVDRYVCNCVIGYIGERCQHRDLRWW KLR
    • 領域
      974 to 1026

特性

Our Abpromise guarantee covers the use of ab123750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    The ED50 as calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of > 1 x 107units/mg.
  • アプリケーション

    HPLC

    Functional Studies

    SDS-PAGE

  • 精製度
    > 95 % SDS-PAGE.
    ab123750 is purified by proprietary chromatographic techniques. The Rat EGF Purity is greater than 98% as determined by HPLC and SDS-PAGE.
  • 製品の状態
    Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at -20ºC.

    pH: 7.40
    Constituent: 99% PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    Centrifuge the vial prior to opening. Reconstitute in sterile H2O to a concentration = 100 µg/ml. This solution can then be diluted into other aqueous buffer.

関連情報

  • 別名
    • Beta urogastrone
    • beta-urogastrone
    • EGF
    • EGF_HUMAN
    • Epidermal growth factor
    • HOMG4
    • OTTHUMP00000219721
    • OTTHUMP00000219722
    • Pro epidermal growth factor
    • URG
    • Urogastrone
    see all
  • 機能
    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • 組織特異性
    Expressed in kidney, salivary gland, cerebrum and prostate.
  • 関連疾患
    Hypomagnesemia 4
  • 配列類似性
    Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • 翻訳後修飾
    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • 細胞内局在
    Membrane.
  • Information by UniProt

Recombinant rat EGF protein (ab123750) 使用論文

ab123750 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab123750.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録