製品の概要

  • 製品名Recombinant Protein A + Protein G
  • タンパク質長Protein fragment

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • タグHis tag N-Terminus
    • 配列の追加情報The molecular weight including the His tag is 59.7 kDa

特性

Our Abpromise guarantee covers the use of ab52213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Protein A+G binds to all IgG subclasses (from various mammalian species).
  • アプリケーション

    SDS-PAGE

    Affinity purification

  • 精製度> 98 % SDS-PAGE.

  • 製品の状態Lyophilised
  • 備考


    This product is a genetically engineered fusion protein that combines the IgG binding profiles of both Protein A and Protein G. Protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. The 6x His-tag on the N-terminus can be used for affinity purification or for protein A/G detection using an anti-His-tag antibody. Non-specific binding regions, including cell wall binding regions, albumin binding regions and other non-specific binding regions have been eliminated from this fusion protein A/G, to ensure the maximum specific IgG binding.

    Protein A+G binds to all IgG subclasses (from various mammalian species).
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Endotoxin level is <0.1 EU/mg of Protein A/G.

  • 再構成Reconstitute in water to a concentration of 5 mg/ml.

関連情報

  • 細胞内局在Protein A: Secreted; cell wall. Protein G: Cell Wall and Secreted

Recombinant Protein A + Protein G (ab52213) 使用論文

This product has been referenced in:

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"