製品の概要

製品の詳細

  • 由来Recombinant
  • 由来Escherichia coli
  • アミノ酸配列
    • 領域32 to 327
    • タグHis tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab52953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.
    Purity: >99% by SDS-PAGE and HPLC analyses.
  • 製品の状態Liquid
  • 備考


    The cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from this protein to ensure the maximum specific IgG binding.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: dH2O
    Endotoxin level is <0.1 EU/mg of Protein A.

関連情報

  • 別名
    • gG-binding protein A
    • Immunoglobulin G binding protein A
    • SPA
    • Staphylococcal protein A
  • 関連性Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • 細胞内局在Secreted; cell wall.

Recombinant Protein A (ab52953) 使用論文

ab52953 has not yet been referenced specifically in any publications.

Product Wall

J'aimerais vous informer que mes collègues responsables de l'intégration des produits travaillent toujours à la recherche d'un collaborateur qui pourrait nous fournir :
- une protéine G (full length sans tag) en compl&ea...

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Je viens d'être informé que:

1) les deux protéines A et G His-taggées ab49807 et ab52953 ne sont malheureusement pas disponibles dans la forme sans tag.

2) nous avons ou pouvons ajouter à notre catalo...

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Je suis toujours en attente d'une réponse pour une proposition de protéine His-tag pouvant fixer le fer ou un métal lourd.

Concernant les protéines A et G, nous avons ab49807 (protéine G, acides aminés...

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Thank you for your enquiry. I can confirm that the molecular weight of the protein and His tag is 39kDa. Unfortunately, we do not have further information regarding the diameter, nor the affinity constant. However, we found that binding to human Ig...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"