The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ED50 range= 1-5 ng/ml, determined by the dose-dependant proliferation of BALB/c 3T3.
% SDS-PAGE. ab73233 is purified by proprietary chromatographic techniques.
Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
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Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitute in sterile 100mM acetic acid and 0.1% BSA not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Upon reconstitution ab73233 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.