Recombinant mouse PDE5A/PDE5 protein (ab80330)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
製品の詳細
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製品名
Recombinant mouse PDE5A/PDE5 protein
PDE5A/PDE5 タンパク質・ペプチド 製品一覧 -
生理活性
Specific Activity: 4630 pmol/min/µg. Assay conditions: 10 mM Tris-HCl, pH7.4, 10 mM MgCl2, 1 mM MnCl2, 200 µM cGMP, 2.5 kU 5' nucleotidase, 37ºC, 20min.
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精製度
> 70 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Mouse
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab80330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
Western blot
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製品の状態
Liquid -
備考
This product was previously labelled as PDE5A
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- 5''-cyclic phosphodiesterase
- CGB PDE
- CGB-PDE
see all -
機能
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. -
組織特異性
Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung. -
パスウェイ
Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1. -
配列類似性
Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains. -
ドメイン
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B. -
翻訳後修飾
Phosphorylation is regulated by binding of cGMP to the two allosteric sites. - Information by UniProt
画像
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Lane 1: ab80330 on 10% SDS-PAGE, Coomassie staining, 5µg.
Lane 2: Protein marker.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab80330 は論文での使用が確認できていません。