製品の概要

法規制情報

製品の詳細

  • 由来
    Recombinant
  • 由来
    Baculovirus infected Sf9 cells
  • アミノ酸配列
    • 生物種
      Mouse

特性

Our Abpromise guarantee covers the use of ab80330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    Specific Activity: 4630 pmol/min/µg. Assay conditions: 10 mM Tris-HCl, pH7.4, 10 mM MgCl2, 1 mM MnCl2, 200 µM cGMP, 2.5 kU 5' nucleotidase, 37ºC, 20min.
  • アプリケーション

    Other

    Functional Studies

    SDS-PAGE

    Western blot

  • 製品の状態
    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • 5''-cyclic phosphodiesterase
    • CGB PDE
    • CGB-PDE
    • CGBPDE
    • cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase
    • cGMP binding cGMP specific 35 cyclic nucleotide phosphodiesterase
    • cGMP binding cGMP specific phosphodiesterase
    • cGMP binding/cGMP specific phosphodiesterase
    • cGMP specific 3'5' cyclic phosphodiesterase
    • cGMP specific phosphodiesterase PDE5A2
    • cGMP specific phosphodiesterase type 5A
    • cGMP-binding cGMP-specific phosphodiesterase
    • cGMP-specific 3''
    • CN5A
    • CN5N
    • PDE 5
    • PDE 5A
    • PDE5
    • Pde5a
    • PDE5A_HUMAN
    • PDE5A1
    • Phosphodiesterase 5A
    • Phosphodiesterase 5A cGMP specific
    • Phosphodiesterase isozyme 5
    see all
  • 機能
    Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
  • 組織特異性
    Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
  • パスウェイ
    Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
  • 配列類似性
    Belongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.
  • ドメイン
    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.
  • 翻訳後修飾
    Phosphorylation is regulated by binding of cGMP to the two allosteric sites.
  • Information by UniProt

画像

  • Lane 1: ab80330 on 10% SDS-PAGE, Coomassie staining, 5µg.
    Lane 2: Protein marker.
  • Anti-PDE5A antibody (ab64179) at 1 µg/ml + Recombinant mouse PDE5A protein (ab80330) at 0.001 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

参考文献

ab80330 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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