Full length protein
Baculovirus infected Sf9 cells
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific Activity: 2127 pmol/min/µg.
Assay conditions: 10 mM Tris-HCl, pH7.4, 10 mM MgCl
2, 1 mM MnCl 2, 200 µM cAMP, 2.5 kU 5' nucleotidase, 37ºC, 20min.
Quantified by 5' nucleotidase cleaving the 5' AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
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Shipped on Dry Ice. Store at -80°C.
Constituents: 20% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
This product is an active protein and may elicit a biological response in vivo, handle with caution.
5''-cyclic phosphodiesterase 4C
cAMP specific 3' 5' cyclic phosphodiesterase 4C
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Expressed in various tissues but not in cells of the immune system.
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
Information by UniProt
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"