由来 Baculovirus infected Sf9 cells
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生理活性 Specific Activity: 2127 pmol/min/µg.
Assay conditions: 10 mM Tris-HCl, pH7.4, 10 mM MgCl 2, 1 mM MnCl 2, 200 µM cAMP, 2.5 kU 5' nucleotidase, 37ºC, 20min.
Quantified by 5' nucleotidase cleaving the 5' AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
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Constituents: 20% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
This product is an active protein and may elicit a biological response in vivo, handle with caution.
5''-cyclic phosphodiesterase 4C
cAMP specific 3' 5' cyclic phosphodiesterase 4C
機能 Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
組織特異性 Expressed in various tissues but not in cells of the immune system.
パスウェイ Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
配列類似性 Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
Information by UniProt
Recombinant mouse PDE4C protein 画像
Recombinant mouse PDE4C protein (ab95475) 使用論文
has not yet been referenced specifically in any publications.
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