製品の概要

製品の詳細

  • 由来Recombinant
  • 由来BHK cells
  • アミノ酸配列
    • 生物種Mouse

特性

Our Abpromise guarantee covers the use of ab39309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

    Western blot

  • 製品の状態Liquid
  • 備考

    Mouse-MMP9 degrades denatured collagen (gelatin), and a range of extracellular matrix components in-vivo. Unlike MMP2, MMP9 is not constituitively produced by most normal cells, although the enzyme is often over expressed by transformed cells, tumor cells, and cells treated with the phorbol ester PMA. An endogenous inhibitor to MMP9, TIMP1, is often complexed with the enzyme in-vivo, but has been removed from this preparation.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, PBS, 250mM Sodium chloride, 0.6 % DMSO, pH 7.4

関連情報

  • 別名
    • 82 kDa matrix metalloproteinase-9
    • 92 kDa gelatinase
    • 92 kDa type IV collagenase
    • CLG 4B
    • CLG4B
    • Collagenase Type 4 beta
    • Collagenase type IV 92 KD
    • EC 3.4.24.35
    • Gelatinase 92 KD
    • Gelatinase B
    • Gelatinase beta
    • GelatinaseB
    • GELB
    • Macrophage gelatinase
    • MANDP2
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
    • Matrix Metalloproteinase 9
    • MMP 9
    • MMP-9
    • MMP9
    • MMP9_HUMAN
    • Type V collagenase
    see all
  • 機能May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • 組織特異性Produced by normal alveolar macrophages and granulocytes.
  • 関連疾患Intervertebral disc disease
    Metaphyseal anadysplasia 2
  • 配列類似性Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻訳後修飾Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • 細胞内局在Secreted, extracellular space, extracellular matrix.
  • Information by UniProt

Recombinant Mouse MMP9 protein 画像

  • All lanes : Anti-MMP9 antibody (ab38898) at 2 µg/ml

    Lane 1 : Natural human MMP9 protein (Proenzyme, monomer) (ab157344)
    Lane 2 : Recombinant Mouse MMP9 protein (ab39309)

    Lysates/proteins at 0.1 µg per lane.


    Performed under reducing conditions.

    This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with anti-MMP9 antibody (ab38898; 2 microgram per mL)  overnight at 4°C. Antibody binding was detected using infrared labelled goat anti-rabbit  (green) antibody (diluted 1:20000) for 1 hour at room temperature before imaging.

Recombinant Mouse MMP9 protein (ab39309) 使用論文

This product has been referenced in:
  • Nighot P  et al. Matrix metalloproteinase 9-induced increase in intestinal epithelial tight junction permeability contributes to the severity of experimental DSS colitis. Am J Physiol Gastrointest Liver Physiol 309:G988-97 (2015). Read more (PubMed: 26514773) »
  • Lluri G  et al. Tissue inhibitor of metalloproteinase-2 (TIMP-2) regulates myogenesis and beta1 integrin expression in vitro. Exp Cell Res 314:11-24 (2008). Read more (PubMed: 17678891) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry.

I am pleased to confirm that these are now available on the online datasheet. Please select the SDS tab near the top left hand side of the datasheet.

Slit3 protein ab116753
http://www.abcam.com/ind...

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Thank you very much for your interest in ab39308.

Although this product can be used for this application, we have not directly tested it, therefore, I can offer a discount off a future purchase if you buy ab39308 now, test it in gelatin gel ...

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Thank you once again for your enquiries regarding our MMP9 proteins.

To follow up, I now have further information regarding the following proteins:

ab39308 MMP9 protein (Human)
ab39309 MMP9 protein (Mouse)
ab41067 MMP9 p...

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Thank you for contacting us.

We do have several MMP proteins in our catalog.

We have for MMP2:

ab39302 (human):Full length Human MMP2 protein (purified from human fibroblast cell culture media). It has not yet been tested...

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Thank you for your enquiry regarding ab39309. I can confirm that ab39309 has been characterized for functional assays, SDS-PAGE and Western blot. We have not tested it in ELISA, neither with any ELISA kit, therefore we cannot be sure if it works with G...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"