製品の概要

製品の詳細

  • 由来
    Recombinant
  • 由来
    Escherichia coli
  • アミノ酸配列
    • 生物種
      Mouse
    • 配列
      Recombinant murine JE, also known as monocyte chemotactic protein (MCP-1), is a 13.8 kDa protein containing 125 amino acid residues. QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN

特性

Our Abpromise guarantee covers the use of ab9901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • エンドトキシン・レベル
    < 0.100 Eu/µg
  • 製品の状態
    Lyophilised
  • 備考
    The biological activity of this product was determined by its ability to chemoattract Balb/c mouse spleen MNCs using a concentration range of 0.1-10.0ng/ml.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

関連情報

  • 別名
    • C-C motif chemokine 2
    • CCL2
    • CCL2_HUMAN
    • Chemokine (C C motif) ligand 2
    • GDCF 2
    • GDCF-2
    • GDCF2
    • HC11
    • HSMCR30
    • JE
    • MCAF
    • MCP 1
    • MCP-1
    • MCP1
    • MGC9434
    • Monocyte chemoattractant protein 1
    • Monocyte chemotactic and activating factor
    • Monocyte chemotactic protein 1
    • Monocyte secretory protein JE
    • SCYA2
    • Small inducible cytokine A2
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je)
    • Small inducible cytokine subfamily A (Cys Cys), member 2
    • Small-inducible cytokine A2
    • SMC CF
    • SMC-CF
    • SMCCF
    see all
  • 機能
    Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • 配列類似性
    Belongs to the intercrine beta (chemokine CC) family.
  • 翻訳後修飾
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • 細胞内局在
    Secreted.
  • Information by UniProt

参考文献

This product has been referenced in:
  • Gallagher JJ  et al. Acute and chronic phases of complex regional pain syndrome in mice are accompanied by distinct transcriptional changes in the spinal cord. Mol Pain 9:40 (2013). Mouse . Read more (PubMed: 23924076) »

See 1 Publication for this product

レビューと Q&A

Abreviews
Application
Western blot
The protein ran at a slightly higher molecular weight than expected (ran around ~16kDa as opposed to the expected 13kDa). It also has fragments around ~10kDa. Literature did not indicate a cleavage site.

The protein is not readily visible in sypro at 100ng load. We visualize it in western blot with goat polyclonal antibody to MCP-1
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Abcam user community

Verified customer

投稿 Sep 03 2014



The reconstitution instructions are lot-dependent. I think I tracked down your order and the lot number you have is GR54881-4, in which case the following instructions apply:

Centrifuge the vial prior to opening. Reconstitute in wate...

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Thank you for your enquiry.

The following antibodies from our catalog have been used with the following antibodies. they should therefore be suitable for use together. When proteins have been used with specific antibodies from the catalog, t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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